Research topics
Immunoreceptor signalling, lipid rafts, transmembrane adaptor proteins, leukocyte surface molecules
Current Grant Support
Ministry of Education, Youth and Sports of the Czech Republic (Center of Molecular and Cellular Immunology, grant No.: 1M0506; project 2B06064 (NPV2); FP6 EU (HYBLIB, NEST, grant No.: 12919)
Most important recent papers
(2003-present; names of the lab members underlined)Baumgartner, M., Angelisová, P., Setterblad, N., Mooney, N., Werling, D., Hořejší, V., Langsley, G.: Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B-lymphocytes. Blood 101: 1874-81, 2003. (PubMed) (PDF)
Brdičková, N., Brdička, T., Angelisová, P., Horváth, O., Špička, J., Hilgert, I., Pačes, J., Simeoni, L., Kliche, S., Merten, C., Schraven, B., Hořejší, V.: LIME: a new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J. Exp. Med. 198: 1453-1462, 2003. (PubMed) (PDF)
Volná, P., Lebduška, P., Dráberová, L., Šímová, S., Heneberg, P., Boubelík, M., Bugajev, V., Malissen, B., Wilson, B.S., Hořejší, V., Malissen, M., Dráber, P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J. Exp. Med. 200: 1001-13, 2004. (PubMed) (PDF)
Stork, B., Engelke, M., Frey, J., Hořejší, V., Hamm-Baarke, A., Schraven, B., Kurosaki, T., Wienands, J.: Grb2 and the non-T cell activation linker NTAL constitute a Ca2+-regulating signal circuit in B lymphocytes. Immunity 21: 681-91, 2004. (PubMed) (PDF)
Tedoldi, S., Paterson, J.C., Hansmann, M.L., Natkunam, Y., Rudiger, T., Angelisova, P., Du, M.Q., Roberton, H., Roncador, G., Sanchez, L., Pozzobon, M., Masir, N., Barry, R., Pileri, S., Mason, D.Y., Marafioti, T., Horejsi, V.: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood 107: 213-21, 2005. (PubMed) (PDF)
Tessarz, A.S., Weiler, S., Zanzinger, K., Angelisova, P., Horejsi, V., Cerwenka, A. Non-T cell activation linker (NTAL) negatively regulates TREM-1/DAP12-induced inflammatory cytokine production in myeloid cells. J. Immunol. 178: 1991-9, 2007. (PubMed)
Meraner, P., Horejsi, V., Wolpl, A., Fischer, G.F., Stingl, G., Maurer, D. Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J. Immunol. 178: 2262-71, 2007. (PubMed)
Kitaura., J, Kawakami, Y., Maeda-Yamamoto, M., Horejsi, V., Kawakami, T. Dysregulation of Src family kinases in mast cells from epilepsy-resistant ASK versus epilepsy-prone EL mice. J Immunol. 178: 455-62, 2007. (PubMed)
Gregoire C, Simova S, Wang Y, Sansoni A, Richelme S, Schmidt-Giese A, Simeoni L, Angelisova P, Reinhold D, Schraven B, Horejsi V, Malissen B, Malissen M. Deletion of the LIME adaptor protein minimally affects T and B cell development and function. Eur. J. Immunol. 37: 3259-69, 2007. (PubMed)
Iwaki S, Spicka J, Tkaczyk C, Jensen BM, Furumoto Y, Charles N, Kovarova M, Rivera J, Horejsi V, Metcalfe DD, Gilfillan AM. Kit- and FceRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells. Cell Signal. 20: 195-205, 2008. (PubMed)
Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H. LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol. 45: 1703-11, 2008. (PubMed)
Lo Monaco E, Sibilio L, Melucci E, Tremante E, Such.nek M, Horejsi V, Martayan A, Giacomini P. HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides. J Immunol. 181: 5442-50, 2008. (PubMed)
Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C. The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol. 182: 603-14, 2008. (PubMed)
Wu W, Slaastad HS, de la Rosa Carrillo D, Frey T, Tjonnfjord GE, Boretti E, Aasheim HC, Horejsi V, Lund-Johansen F. Antibody array analysis with label-based detection and resolution of protein size. Mol Cell Proteomics. 2008 Sep 16. [Epub ahead of print] (PubMed)
Reviews
Hořejší, V.: The roles of membrane microdomains (rafts) in T cell activation. Immunol. Rev. 191: 148-163, 2003. (PubMed) (PDF)
Hořejší, V., Zhang, W., Schraven, B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat. Rev. Immunol. 4: 603-16, 2004. (PubMed) (PDF)
Hořejší, V.: Lipid rafts and their roles in T-cell activation. Microbes Infect. 7: 31-16, 2005. (PubMed) (PDF)
Other results
Useful monoclonal antibodies (link)
Teaching, lectures, public presentations
Immunology courses, Faculty of Science, 3rd Faculty of Medicine, Charles University, Prague; presentation for Czech students (link)