Journal of Biological Chemistry

N- terminal loop and wing W2- regions of forkhead domain are important for stability of the FoxO4-DBD-DNA complex.

FoxO4 belongs to the "O" subset of forkhead transcription factors, which participate in various cellular processes. The forkhead DNA binding domain (DBD) consists of three-helix bundle resting on a small antiparallel beta-sheet from which two extended loops protrude and create two wing-like structures. In this report, we investigated the role of the N-terminal loop and the C-terminal region (loop known as wing W2) of the forkhead domain of transcription factor FoxO4 in DNA binding. Forster resonance energy transfer measurements and molecular dynamics simulations suggest that both studied N- and C-terminal regions of FoxO4-DBD directly interact with DNA. Our results show that both N- and C-terminal regions of forkhead domain are important for stability of the FoxO4-DBD-DNA complex.
Boura E, Silhan J, Herman P, Vecer J, Sulc M, Teisinger J, Obsilova V, Obsil T: Both the N-terminal loop and wing W2 of the forkhead domain of transcription factor FoxO4 are important for DNA binding. Journal of Biological Chemistry. 2007; 282(11): 8265-8275 IF=5,581.

Author: obsilovabiomed.cas.cz

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