Research topics

Modulation of microtubule properties by signal transduction molecules, function of gamma-tubulin forms, microtubule-associated proteins, interactions between microtubules and other cellular components.

Current grant support

Ministry of Education, Youth and Sports of the Czech Republic (Center of Cell Functional Organization, grant No.: LC545)
Grant Agency of the Czech Republic (grants Nos.: 204/09/1777, 204/09/H023)
Grant Agency of the Academy of Sciences of the Czech Republic (KAN200520701)

Most important recent papers

(2003-present; names of the lab members underlined)

Dryková, D., Sulimenko, V., Cenklová, V., Volc, J., Dráber, P., Binarová, P.: Plant gamma-tubulin interacts with alpha/beta-tubulin dimers and forms membrane-associated complexes. Plant Cell 15: 465-480, 2003. (PubMed)

Porchet, R., Probst, A., Bouras, C., Dráberová, E., Dráber, P., Riederer, B.M.: Analysis of GFAP in the human entorhinal cortex during aging and in Alzheimer's disease. Proteomics 3: 1476-1485, 2003. (PubMed)

Porchet, R., Probst, A., Dráberová, E., Dráber, P., Riederer, I.M., Riederer, B.M.: Identification of modified neurofilament proteins in the human entorhinal cortex with monoclonal antibodies. NeuroReport 14: 929-933, 2003. (PubMed)

Libusová, L., Sulimenko, T., Sulimenko, V., Hozák, P., Dráber, P.: Gamma-tubulin in Leishmania: cell-cycle dependent changes in subcellular localization and heterogeneity of its isoforms. Exp. Cell Res. 295: 375-386, 2004. (PubMed)

Sulimenko, T., Dráber, P.: A fast and simple dot-immunobinding assay for quantification of mouse immunoglobulins in hybridoma culture supernatants. J. Immunol. Methods 289: 89-95, 2004. (PubMed)

Kukharskyy V., Sulimenko V., Macůrek L., Sulimenko T., Dráberová E., Dráber P.: Complexes of gamma-tubulin with non-receptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp. Cell Res. 298: 218-228, 2004. (PubMed)

Katsetos, C.D., Reddy, G., Dráberová, E., Šmejkalová, B., Del Valle, L., Ashraf, Q., Tadevosyan, A., Yelin, K., Maraziotis, T., Mishra, O.P., Mörk, S., Legido, A., Nissanov, J., Baas, P.W., de Chadarévian, J.P., Dráber P.: Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines suggest centrosome protein amplification. J. Neuropathol. Exp. Neurol. 65: 465-477, 2006. (PubMed)

Sulimenko, V.,Dráberová, E., Sulimenko, T., Macůrek, L., Richterová, V., Dráber, Pe., Dráber, P.: Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J. Immunol. 176: 7243-7253, 2006. (PubMed)

Pěknicová, J., Pexidrová, M., Kubátová, A., Koubek, P., Teplá, O., Sulimenko, T., Dráber, P., Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil. Steril. 88: 1120-1128, 2007. (PubMed)

Katsetos, C.D., Dráberová, E., Šmejkalová, B., Reddy, G., Bertrand, L., Chadarévian, J.P., Legido, A., Nissanov J., Baas, P.W., Dráber, P.: Class III ß-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells. Neurochem. Res. 32: 1387-1398, 2007. (PubMed)

Dráberová, E., Del Valle, L., Gordon, J., Marková, V., Šmejkalová, B., Bertrand, L., de Chadarévian, J.P., Agamanolis, D.P., Legido, A., Khalili, K., Dráber, P., Katsetos, C.D.: Class III beta-tubulin is constitutively co-expressed with GFAP and nestin in midgestational human fetal astrocytes in primary culture: comparative observations in the fetal brain and implications in phenotypic identity. J. Neuropathol. Exp. Neurol. 67: 341-354, 2008. (PubMed)

Blume, Y., Yemets A., Sulimenko, V., Sulimenko, T., Chan, J., Lloyd, C., Dráber, P: Tyrosine phosphorylation of plant tubulin. Planta 229: 143-150, 2008. (PubMed)

Macůrek, L., Dráberová, E., Richterová, V., Sulimenko, V., Sulimenko, T., Dráberová, L., Marková, V., Dráber, P. Regulation of microtubule nucleation in differentiating embryonal carcinoma cells by complexes of membrane-bound gamma-tubulin with Fyn kinase and phosphoinositide 3-kinase. Biochem. J. 416: 421-430, 2008. (PubMed)

Reviews

Binarová, P., Cenklová, V., Pochylová, Ž., Dráberová, E., Dráber, P.: Gamma-tubulins and their functions in plant cells. In: The Plant Cytoskeleton. Eds: Y.B. Blume V.W. Baird, A.I. Yemets. D. Breviario. Springer Verlag, ISBN: 987-1-4020-8841-4, pp 23-43, 2008.

Other results

Monoclonal antibodies against cytoskeletal proteins (link) + (Exbio).

Teaching, lectures, public presentations