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Na+/H+ exchange in glial cells of Necturus optic nerve.
Astion ML, Chvatal A, Orkand RK
Institute of Neurobiology, University of Puerto Rico Medical Sciences, San Juan 00901.
Single and double-barreled pH-sensitive electrodes were used to study intracellular pH (pHi) regulation in glial cells of Necturus optic nerve in the nominal absence of HCO3-/CO2. After the cells were acidified by the addition and withdrawal of NH4+, the pHi recovered toward the original steady-state pHi. The recovery from acidification was Na+-dependent and inhibited by 1 mM amiloride. These results suggest the existence in intact vertebrate glial cells of a Na+/H+ exchanger which functions in acid extrusion.
