Research Topics
Retroviruses, retroviral vectors, receptors for retroviruses, latency and persistence of retroviruses, endogenous retroviruses, retrotransposons, epigenetics, src-oncogene, transgenesis in chicken.
Current grant support
Ministry of Education, Youth and Sports of the Czech Republic (Center of Cellular Invasiveness in Embryonic Development and Tumour Metastasis, grant No.: LC06061)
Grant Agency of the Czech Republic (grants Nos: 204/07/1030, 523/07/1171, 523/07/1282, 204/08/P616, 301/09/2031, 301/09/P667)
Academy of Sciences of the Czech Republic (grant No: A500520709)
European Committee (6th FP Project XENOME).
Most important recent papers
(2004-present; names of the lab members underlined)Elleder, D., Plachý, J., Hejnar, J., Geryk, J., Svoboda, J.: Close linkage of genes encoding receptors for subgroups A and C of avian sarcoma/leukosis virus on chicken chromosome 28. Anim. Genet. 35: 176-181, 2004. (PubMed)
Elleder, D., Melder, D.C., Trejbalova, K., Svoboda, J., Federspiel, M.: Two different molecular defects in the Tva receptor gene explain the resistance of two tva lines of chickens to infection by subgroup A avian sarcoma and leukosis viruses. J. Virol. 78: 13489-13500, 2004 (PubMed) (PDF)
Elleder, D., Stepanets, V., Melder, D.C., Šenigl, F., Geryk, J., Pajer, P., Plachý, J., Hejnar, J., Svoboda, J., Federspiel, M., J.: The receptor for the subgroup C avian sarcoma and leukosis viruses, Tvc, is related to mammalian butyrophilins, members of the immunoglobulin superfamily. J. Virol. 79: 10408-10419, 2005. (PubMed)
Matoušková, M., Blažková, J., Pajer, P., Pavlíček, A., Hejnar, J.: CpG methylation suppresses transcriptional activity of human syncytin-1 in non-placental tissues. Exp. Cell Res., 312: 1011-20, 2006. (PubMed)
Trefil P, Micakova A, Mucksova J, Hejnar J, Poplstein M, Bakst MR, Kalina J, Brillard JP.: Restoration of spermatogenesis and male fertility by transplantation of dispersed testicular cells in the chicken. Biol. Reprod. 75: 575-581, 2006. (PubMed)
Prukova D, Vernerova Z, Pilcik T, Stepanets V, Indrova M, Geryk J, Plachy J, Hejnar J, Svoboda J.:Differences in pathogenicity among strains of the same or different avian leukosis virus subgroups. Avian Pathol. 36: 15-27, 2007. (PubMed)
Kalina J, Šenigl F, Mičáková A, Mucksová J, Blažková J, Poplštein M, Hejnar J, Trefil P: Retrovirus-mediated in vitro gene transfer into chicken male germ line cells. Reproduction 134: 445-453, 2007. (PubMed)
Reinišová M, Šenigl F, Yin XQ, Plachý J, Geryk J, Elleder D, Svoboda J, Federspiel MJ, Hejnar J: A Single-Amino-Acid Substitution in the TvbS1 Receptor Results in Decreased Susceptibility to Infection by Subgroups B and D and Resistance to Infection by Subgroup E Avian Sarcoma and Leukosis Viruses In Vitro and In Vivo. J. Virol. 82: 2097-2105, 2008. (PubMed)
Reinišová M, Pavlíček A, Divina P, Geryk J, Plachý J, Hejnar J: Target site preference of subgroup C Rous sarcoma virus integration into the chicken DNA. The Open Genomics J. 1: 6-12, 2008. (FullText)
Šenigl F, Plachý J, Hejnar J: The core element of a CpG island protects retroviral vectors from transcriptional silencing. J. Virol. 82: 7818-7827, 2008. (PubMed)
Reviews
Svoboda J: Foundations in cancer research. The turns of life and science. Adv. Cancer Res. 99: 1-32, 2008. (PubMed)
Other results
Application of a patent on regulatory element protecting retroviral vectors from CpG methylation and transcriptional silencing.
Teaching, lectures, public presentations
The course of Experimental Oncology by Jan Svoboda, Faculty of Science, Charles University, Prague.