Transporters in cell physiology

Abstracts

2008

• Zahrádka J., Flegelová-Kučerová H., Sychrová H.: GFP tagging influences the biogenesis and activity of the yeast Nha1 Na+/H+ antiporter.
  Sborník XII. Setkání biochemiků a molekulárních biologů, Brno, p. 59 (2008).
• Elicharová H., Sychrová H.: Development of genetic tools for the osmotolerant yeast Debaryomyces hansenii.
  Sborník XII. Setkání biochemiků a molekulárních biologů, Brno, p. 67 (2008).
• Petrezselyova S., Herynkova P., Zahradka J. Sychrova H.: Construction and phenotypic characterization of mutants lacking plasma membrane alkali-metal-cation transport systems.
  Abstracts of the III Translucent Workshop: Gene interaction networks and models of cation homeostasis in Saccharomyces cerevisiae. Cordoba, p. 14 (2008).
• Krauke, Y., Zimmermannova O., Sychrova H.: Plasma membrane Na+/H+ antiporters contribute to the salt tolerance of pathogenic Candida species.
  Abstracts of ASM 9th Conference on Candida and Candidiasis, Jersey City, p. 44-45 (2008).
• Krauke, Y., Zimmermannova O., Sychrova H.: Role of CgCnh1 antiporter in tolerance of Candida glabrata to alkali metal cations.
  Abstracts of ASM 9th Conference on Candida and Candidiasis, Jersey City, p. 46-47 (2008).
• Krauke, Y., Zimmermannova O., Sychrova H.: Salt tolerance of Candida species reflects the aktivity of alkali-metal-cation/H+ antiporters.
  Abstracts of the 36th Annual Conference on Yeasts, Smolenice, p 37 (2008).
• Petrezselyova S., Herynkova P., Sychrova H.: Phenotypic characterization of Saccharomyces cerevisiae mutants lacking plasma membrane K+-specific transport systems.
  Abstracts of the 36th Annual Conference on Yeasts, Smolenice, p 83 (2008).
• Kraidlova L., Maidan M. M., Sychrova H., van Dijck P.: GAP permeases in Candida albicans.
  Abstracts of the PhD Student Workshop of Institute of Physiology, Physiol. Res 57, 79P (2008).
• Krauke Y., Zimmermannova O., Sychrova H.: Role of Na+/H+ antiporters in salt tolerance of Candida species.
  Abstracts of the PhD Student Workshop of Institute of Physiology, Physiol. Res 57, 80P (2008).
• Petrezsélyová S., Herynková P., Zahrádka J., Sychrová H.: Construction and phenotype study of S. cerevisiae strains lacking K+ transporters.
  Annual SysMo Conference, Bad Honnef, p 18 (2008).
• Sychrova, H., Krauke, Y. , Papouskova, K., Pribylova, L., Zimmermannova O.: Yeast Na+/H+ antiporters play an important role in potassium homeostasis.
  2008 Yeast Genetics and Molecular Biology Meeting, Toronto, p. 151 (2008).
• Pribylova L., Sychrova H.: Tools for genetic manipulation of the osmotolerant yeast Zygosaccharomyces rouxii.
  12th International Congress on Yeasts, Kyjev, p. 108 (2008).
• Maresova L., Sychrova H.: Plasma membrane cation transporters influence the membrane potential of Saccharomyces cerevisiae.
  12th International Congress on Yeasts, Kyjev, p. 177 (2008).
• Sychrova H.: Potassium and sodium transporters in yeast.
  Abstracts of the 26th SMYTE, Braga, p. 26 (2008).
• Pribylova L., Papouskova K., Sychrova H.: Osmotolerant yeast Z. rouxii possess two plasma-membrane Na+/H+ antiporters to ensure salt tolerance and intracellular potassium homeostasis.
  Abstracts of the 26th SMYTE, Braga, p. 75 (2008).
• Maresova L., Sychrova H., Rao R.: Membrane hyperpolarization drives fungicidal activity of amiodarone.
  Abstracts of the 26th SMYTE, Braga, p. 54 (2008).
• Petrezselyova S., Herynkova P., Sychrova H.: Trk2 plays a possible physiological role under respiratory conditions.
  Abstracts of the 26th SMYTE, Braga, p. 77 (2008).
• Petrezselyova S., Herynkva P., Sychrova H.: K+ transport cez plazmatickú membránu Saccharomyces cerevisiae.
  Zborník abstraktov: História, súčasnosť a perspektívy genetiky. ISBN 978-80-223-2413-7, Bratislava, str. 65 (2008).
• Zahrádka J. , Flegelova-Kucerova H., Sychrova H.: Biogenesis and activity of the yeast Nha1 Na+/H+ antiporter tagged by GFP.
  Abstracts of the Fifth Meeting of Biomedical Doctoral Schools, Strasbourg, p. 35 (2008).
• Elicharova H.,Sychrova H.: Vývoj nástrojů genové manipulace pro osmotolerantní kvasinku Debaryomyces hansenii.
  XVI. Ročník studentské vědecké konference, Farmaceut. fakulta UK, Hradec Králové (2008).

2007

• Zimmermannova O., Papouskova K., Pribylova L., Sychrova H.: Na+/H+ antiporters from osmotolerant yeast species increase the salt tolerance of Saccharomyces cerevisiae. Abstracts of the 35th Annual Conference on Yeasts, Smolenice, p 60 (2007).
• Krauke Y., Zimmermannova O., Sychrova H.: Role of Na+/H+ antiporters in Candida species. 107th General Meeting of American Society for Microbiology, Toronto, 043K-028 (2007).
• Zimmermannova O., Papouskova K., Pribylova L., Sychrova H.: Expression of Na+/H+ antiporters from osmotolerant yeast species improves the tolerance of Saccharomyces cerevisiae. Abstracts of the 26th International Specialized Symposium on Yeast, Sorrento, p. 157 (2007).
• Krauke, Y., Zimmermannova O., Sychrova H.: Do Cnh1 antiporters from different Candida species have the same function? Abstract of the Fourth Meeting of Biomedical Doctoral Schools, Prague, p. 12 (2007).
• Krauke, Y., Zimmermannova O., Sychrova H.: First characterization of the Cnh1 antiporter function in Candida species. Abstract of the Second FEBS Advanced Lecture Course “Human Fungal Pathogens”, La Colle sur Loupe, p.76 (2007).
• Krauke, Y., Zimmermannova O., Sychrova H.: Functional comparison of Cnh1 antiporters from different Candida species. Abstract of the 32nd FEBS Congress “Molecular Machines”, Vienna, p. 127 (2007).
• Zimmermannova O., Sychrova H.: Yeast plasma membrane Na+/H+ antiporter – importance of hydroxyl groups in the fifth transmembrane domain for the transport activity and substrate specificity. Abstract of the 32nd FEBS Congress “Molecular Machines”, Vienna, p. 129 (2007).
• Maresova, L., Sychrova, H.: Studying the function of yeast cation transporters with various microplate reader techniques. Abstracts of the 7th Young Scientist Forum Molecular Networks, Vienna, Austria, p. 60 (2007).
• Maresova, L., Sychrova, H.: The function of yeast cation transporters studied by various microplate reader techniques. Abstracts of the 32nd FEBS Congress. Vol. 274 Vienna, Austria: The FEBS Journal, p. 124 (2007).
• Maresova, L., Urbankova, E., Sychrova, H.: Regulation of yeast cation homeostasis by potassium transporters. Abstracts of the Yeast Cell Biology, Cold Spring Harbor Laboratory, New York, USA, p. 80 (2007).
• Kraidlova L., Maidan M. M., Sychrova H., van Dijck P.: Role of CaGAP permeases. Abstracts of the 32nd FEBS Congress: Molecular Machines, Vienna, The FEBS Journal, p. 117 (2007).
• Kraidlova L., Maidan M. M., Sychrova H., van Dijck P.: Role of CaGAP permeases. Abstracts of 7th FEBS Young Scientist Forum - YSF2007, Vienna, p. 54 (2007).
• Kraidlova L., Maidan M. M., Sychrova H., van Dijck P.: Substráte specificity and function of Candida albicans general amino-acid permeases (CaGaps). Abstracts of the Second FEBS Advanced Lecture Course Human Fungal Pathogens: Molecular Mechanisms of Host-Pathogen Interactions and Virulence, La Colle sur Loup, p. 71 (2007).
• Zimmermannova O., Papouskova K., Pribylova L., Sychrova H.: Heterologous expression of Na+/H+ antiporters from Zygosaccharomyces rouxii and Yarrowia lipolytica increases the salt tolerance of Saccharomyces cerevisiae. Abstrakty 24. kongresu Československé společnosti mikrobiologické, p. 91, Liberec (2007).
• Sychrova H.: Role of Na+/H+ antiporters in yeast physiology. Abstracts of the 25th SMYTE, Arraial d’Ajuda, p. 27 (2007).
• Krauke, Y., Zimmermannova O., Sychrova H.: Plasma-membrane Na+/H+ antiporters in Candida species. Abstracts of the 25th SMYTE, Arraial d’Ajuda, p. 41 (2007).
• Pribylova, L., Papouskova K., de Montigny, J., Sychrova, H: Zygosaccharomyces rouxii possesses two  Na+/H+ antiporters with different substrate specificity. Abstracts of the Yeast Cell Biology Meeting, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA, p. 81. (2007).
• Pribylova, L., Papouskova, K., de Montigny, J., Sychrova, H.: Characterization of  Zygosaccharomyces rouxii Na+/H+ antiporters. Abstracts of the 32nd FEBS Congress Molecular Machines, Vienna, Austria. FEBS J 274 Suppl 1, p. 127 (2007).
• Kraidlova L., Sychrova H., van Dijck P.: Role of CaGAP permeases in the virulence and pathogenicity of Candida albicans. Abstracts of Conference Molecular Mechanisms of Cell Interactions, Prague, p. 10 (2007).
• Vinterová Z., Dostál J., Flegelová H., Pichová I., Sychrová H., Hrušková-Heidingsfeldová O.: Rozvoj metod pro studium sekreční dráhy proteázy Sapp1p kvasinky Candida parapsilosis. 4. Česko-slovenská mezioborová konference lékařské mykologie, Pardubice, p. 73. (2007).

2006

• Kinclova-Zimmermannova 0., Sychrova H.: The Candida albicans CNH1 gene encodes an antiporter important for potassium and pH homeostasis. Abstracts of 8th Candida and Candidiasis, 50, Denver (2006).
• Kinclova-Zimmermannova 0., Sychrova H.: The Cnh1 antiporter is important for potassium and pH homeostasis in C. albicans cells. Abstracts of the 34th Annual Conference on Yeasts, 81, Smolenice (2006).
• Pribylova L., de Montigny J., Sychrova H.: Genetic manipulation in the osmotolerant yeast Zygosaccharomyces rouxii. Abstracts of the 34th Annual Conference on Yeasts, 80, Smolenice (2006).
• Maresova L., Sychrova H.: Complementation of S. cerevisiae KHA1 deletion phynotypes by a plant antiporter AtChx17. Abstracts of the 34th Annual Conference on Yeasts, 77, Smolenice (2006).
• Papouskova K., Sychrova H.: Yast plasma membrane alkali metal cation/H+ antiporters: A phylogenetic study. Abstracts of the 34th Annual Conference on Yeasts, 64, Smolenice (2006).
• Kinclova-Zimmermannova O., Zavrel M., Sychrova H.: Amino-acid residues involved in determination of substrate specificity of yeast plasma membrane Na⁺/H⁺ antiporters. Abstracts of the 34th Annual Conference on Yeasts, 35, Smolenice (2006).
• Marešová, L., Urbánková, E.Gášková, D., Sychrová, H.: The influence of potassium transport systems on the plasma membrane potential of S. cerevisiae cells. Abstracts of the 3rd International Conference on Structure, Dynamics and Function of Proteins in Biological Membranes, 105, Ascona (2006).
• Zimmermannová, O., Sychrová, H.: Amino-acid residues involved in determination of substrate specificity of yeast plasma membrane Na⁺/H⁺ antiporters. Abstracts of the 3rd International Conference on Structure, Dynamics and Function of Proteins in Biological Membranes, 106, Ascona (2006).
• Kinclova-Zimmermannova O., Papouskova K., Maresova L., Sychrova H.: Multiple functions of Na/H antiporters in yeast. Abstracs of ISSY25 – Systems Biology of Yeasts, 159, Helsinki (2006).
• Papouskova K, Sychrova H.: Heterologous expression of two Yarrowia lipolytica NHA genes in Saccharomyces cerevisiae cells. Abstracts of 10th International Symposium in the Genetics of Industrial Microorganisms, 73, Prague (2006).
• Flegelova H., Haguenauer-Tsapis R., Sychrova H.: Saccharomyces cerevisiae Nha1 antiporter is a very stable plasma membrane protein. Abstracts of the 2006 Yeast Genetics and Molecular Biology Meeting, 92, Princeton (2006).
• Zimmermannova O., Zavrel M., Sychrova H.: Identification of amino-acid residuem of the fifth transmembrane domain important for the substrate specificity of trast plasma membráně Na/H antiporters. Abstracts of the 2006 Yeast Genetics and Molecular Biology Meeting, 140, Princeton (2006).
• Pribylova L., de Montigny J., Sychrova H.: Development of tools for genetic manipulation in the osmotolerant yeast Zygosaccharomyces rouxii. Abstracts of the 2006 Yeast Genetics and Molecular Biology Meeting, Princeton, p. 208 (2006).
• Vinterová Z., dostál J., Flegelová H., Pichová I., Sychrová H., Hrušková-Heidingsfeldová O.: The preparation of suitable methods for study of the secretion pathway of proteinase Sapp1pp of the pathogenic yeast Candida parapsilosis. Abstracts of the 24th SMYTE, Prague, p. 52 (2006).
• Hasenbrink G., Kolacna L., Ludwig J., Sychrova H., Kschischo M., Lichtenberg-Fraté H.: Ring test assesssmentof the mKir2.1 growth based assay in saccharomyces cerevisiae using parametric models and model free fits. Abstracts of the 24th SMYTE, Prague, p. 39 (2006).
• Zimmermannová, O., Sychrová, H.: Importance of the seryl and threonyl residues of the fifth transmembrane domain to the substrate specificity of yeast plasma membrane Na+/H+ antiporters. Abstracts of the 24th SMYTE, Prague, p. 15 (2006).
• Krauke Y., Zimmermannová, O., Sychrová, H.: Characterization of Cnh1 antiporters in different Candida species. Abstracts of the 24th SMYTE, Prague, p. 43 (2006).
• Marešová, L., Urbánková, E., Gášková, D., Sychrová, H.: Potassium transport systems and plasma membrane potential of S. cerevisiae cells. Abstracts of the 24th SMYTE, Prague, p. 45 (2006).
• Papouskova K, Sychrova H.: Characterization of Nha antiporters from three non-related yeast species. Abstracts of the 24th SMYTE, Prague, p. 46 (2006).
• Pribylova L., Papouskova K., de Montigny J., Sychrova H.: Na+/H+ antiporters of Zygosaccharomyces rouxii. Abstracts of the 24th SMYTE, Prague, p. 48 (2006).

2005

• Urbánková, E. Marešová, L., Gášková, D., Sychrová, H.: Plasma membrane potential of S. cerevisiae cells and potassium transport systems. Abstracts of the 33rd Annual Conference on Yeasts, Smolenice, p 48 (2005).
• Velková K., Sychrová H.: Kvasinkové systémy pro transport kationtů alkalických kovů. V. Mezioborové setkání mladých biologů, biochemiků a chemiků, Chem. Listy 99, p. 390 (2005).
• Zimmermannová 0., Sychrová H.: Functional study of the Na,K/H antiporter in the pathogenic yeast Candida albicans. Abstracts of the FEBS Advanced Lecture Course HFP, p. 39, La Colle-sur-Loup (2005).
• Flegelova H., Haguenauer-Tsapis R., Sychrova H.: The Nha1 antiporter from the Saccharomyces cerevisiae is an extremely stable plasma membrane protein. Yeast 22, S120. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Pribylova L., de Montovny J., Potier S., Sychrova H.: Improving genetic tools to direct gene cloning in the osmoresistant yeast Zygosaccharomyces rouxii. Yeast 22, S90. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Maresova L., Sychrova H.: S. cerevisiae Kha1p is not a plasma membrane K/H antiporter. Yeast 22, S177. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Malinska K., Sychrova H.: Targeting of the S. cerevisiae Na/H antiporter Nha1 to the plasma membrane depends on the final step in ergosterol biosynthesis. Yeast 22, S177. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Velkova K., Sychrova H.: Isolation of the NHA1 gene from two Debaryomyces hansenii strains. Yeast 22, S178. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Zimmermannova O., Zavrel M., Sychrova H.: Identification of amino-acid residues important for the substrate specificity range of yeast plasma membrane Na/H antiporters. Yeast 22, S179. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Hasenbrink, G., Schwarzer, S., Kolacna, L., Ludwig, J., Sychrova, H., Lichtenberg-Frate, H.:   Analysis of the mKir2.1 channel aktivity in potassium influx defective Saccharomyces cerevisiae strains determined as changes in growth characteristics. Yeast 22, S185. Abstracts of the XXIInd  International Conference on Yeast Genetics and Molecular Biology, Bratislava (2005).
• Malinska K., Sychrova H.: S. cerevisiae Nha antiporter is a raft protein and its targeting to the plasma membrane depends in the last step of ergosterol biosynthesis. Proceedings of the 23rd SMYTE, p. 17, New Delhi (2005).
• Schwartzer S., Sychrova H., Lichtenberg-Frate H., Ludwig J.: Functional expression and analysis of the Kir3.2 K-channel in Saccharomyces cerevisiae. Proceedings of the 23rd SMYTE, p. 59, New Delhi (2005).
• Lichtenbeg-Frate H., Hasenbrink G., Kolacna L., Schwartzer S., Ludwig J., Sychrova H.: Analysis of the mKir2.1 channel aktivity in porassium influx and efflux defective Saccharomyces cerevisiae strains. Proceedings of the 23rd SMYTE, p. 62, New Delhi (2005).
• Maresova, L , Urbankova, E., Gaskova, D., Sychrova, H.: Measuring of plasma membrane potential changes in S. cerevisiae cells by the DiS-C3(3) assay reveals the importance of the Tok1 channel in membrane potential maintenance. XXIV International Specialized Symposium On Yeasts, p. 181, Oropesa del Mar (2005).
• Pribylova L., Sychrova H.: Zygosaccharomyces rouxii - osmotolerant and cell wall properties of the two mostly studied wild-type strains (CBS 732 and ATCC 42981). XXIV International Specialized Symposium On Yeasts, p. 75, Oropesa del Mar (2005).
• Flegelova H., Kolacna L., Zimmermannova O., Sychrova H.: Yeast as a host to study mammalian Na+/H+ antiporters and potassium channels. Abstracts of The First Congress of Physiological Sciences of Serbia and Montenegro, p. 41, Belgrade (2005).
• Velkova K., Sychrova H.: Potassium efflux – a conserved ability of yeast alkali-metal-cation/H+ antiporters? Abstracts of Conference Molecular Mechanisms of Cell Interactions, p. 29, Nove Hute (2005).

2004

• Kinclová-Zimmermannová O., Sychrová H.: Involvement of the Saccharomyces cerevisiae plasma membrane Na+/H+-antiporter Nha1p in the regulation of intracellular cation homeostasis and in cell response to hyperosmotic shock. Abstracts of the EMBO/HHMI Central European Scientists Meeting, p. 18, Prague (2004).
• Koláčná L., Sychrová H.: Functional expression of mammalian Kir2.1 potassium channel in yeast. Proceedings of the 80th Conference of Czech and Slovak Physiological Societies, Phys. Research 53, P16 (2004).
• Koláčná L., Sychrová H.: Yeast as a model for mammalian potassium channel expression. Abstracts of the 32nd Annual Conference on Yeasts, p. 45, Smolenice (2004).
• Kinclová-Zimmermannová O., Sychrová H.: Saccharomyces cerevisiae Na+/H+-antiporter Nha1p participates in the regulation of intracellular alkali-metal-cation homeostasis and in cell response to sudden changes of external osmolarity. Abstracts of the 32nd Annual Conference on Yeasts, p. 43, Smolenice (2004). 
• Flegelova H., Sychrova H.:  Yeast as a model for characterization of mammalian Na+/H+ antiporters. Abstracts of the 32nd Annual Conference on Yeasts, p. 38, Smolenice (2004).
• Maresova, L., Sychrova, H.: Localization and function of the putative K+/H+ antiporter Kha1. Abstracts of the 32nd Annual Conference on Yeasts Smolenice, p. 19, Smolenice (2004).
• Maresova, L., Sychrova H.: Studium transportních proteinů v Sachcaromyces cerevisiae. Abstrakty Studentské vědecké konference 2.LF UK v Praze, p. 13, Praha (2004).
• Pribylova, L., de Montigny, J., Potier, S. and Sychrova, H.: Physiological properties of the osmotolerant yeast Zygosaccharomyces rouxii. Abstracts of the 32nd Annual Conference on Yeasts, Smolenice, p. 26 (2004).
• Velkova, K., Pribylova, L. and Sychrova, H.: Na+/H+ antiporters in different yeast species structure and function comparison. Abstracts of the 32nd Annual Conference on Yeasts, Smolenice, p 84 (2004).
• Pribylova, L. and Sychrova, H. : Tvorba nástrojů genového inženýrství pro cílenou disrupci a deleci genů osmotolerantní kvasinky Zygosaccharomyces rouxii. Chemicke Listy 98, p. 300 (2004).
• Hasenbrink, G., Kolacna, L., Schwarzer, S., Ludwig, J., Sychrova, H., and Lichtenberg-Fraté, H.: Phenotypic analysisof the mKir2.1 channel activity in potassium influx and efflux defective Saccharomyces cerevisiae strains. Abstracts of the Yeast Genetics and Molecular Biology Meeting, p.131, Seattle (2004).
• Zimmermannova O, Sychrova H.: Functional study of the Na,K/H – antiporter in the pathogenic yeast Candida albicans. Proceedings of the 22th SMYTE, p. 23, Ponta Delgada (2004).
• Maresova L., Sychrova H.: Characterization of the putative K/H antiporter Kha1. Proceedings of the 22th SMYTE, p. 24, Ponta Delgada (2004).
• Zimmermannova O., Zavrel M., Sychrova H.: Random mutagenesis of Zygosaccharomyces rouxii Na/H-antiporter Sod2-22p revealed amino-acid residues important for the substrate specificity range. Proceedings of the 22th SMYTE, p. 25, Ponta Delgada (2004).
• Flegelova H., Sychrova H.: Characterization of mammalian NHE antiporters upon their expression in Saccharomyces cerevisiae. Proceedings of the 22th SMYTE, p. 26, Ponta Delgada (2004).
• Pribylova L., Sychrova H.: Wild type strains of the yeast Zygosaccharomyces rouxii (CBS 732 and ATCC 42981) differ in osmotolerance and other properties. Abstracts of Halophiles 2004, p. 35, Ljubljana (2004).
• Pribylova L., de Montigny J., Potier S., Sychrova H.: Properties of the osmotolerant yeast Zygosaccharomyces rouxii. Abstracts of the 2nd Meeting of Doctoral Schools of the Charles University and University Louis Pasteur, p. 9, Prague (2004).
• Velková K., Sychrová H.: Characterization of Na/H antiporters from non-conventional yeasts upon their heterologous expression in Saccharomyces cerevisiae. Abstracts of the Conference of PhD Students, p. 29, Prague (2004).
• Pribylova L., de Montigny J., Potier S., Sychrova H.: Characterization of Zygosaccharomyces rouxii, an extremely osmotolerant yeast species. Abstracts of the Conference of PhD Students, p. 26, Prague (2004).