REACTIVE OXYGEN SPECIES (ROS) GENERATED BY XANTHINE OXIDASE IN THE CORNEAL EPITHELIUM AND THEIR POTENTIAL PARTICIPATION IN THE DAMAGE OF THE CORNEAL EPITHELIUM AFTER PROLONGED USE OF CONTACT LENSES IN RABBITS
Chemical abstracts: 128:281330
Cejkova J., Labsky J., Vacik J. (Department Eye Histochemistry, Institute Experimental Medicine, Academy Sciences Czech Republic, Prague 14220, Czech Rep.). Acta Histochem., 100(2), 171-184 (English) 1998 Gustav Fischer Verlag CODEN: AHISA9. ISSN: 0065-1281. DOCUMENT TYPE: Journal CA Section: 14 (Mammalian Pathological Biochemistry)
The effect of reactive O species (ROS) generated by xanthine oxidase to corneal damage after prolonged use (14 days) of contact lenses was investigated in eyes of rabbits. An imbalance was evoked between the activity of xanthine oxidase and catalase in the corneal epithelium. Catalase activity was decreased and xanthine oxidase activity was very high. The activities of xanthine oxidoreductase, glucose-6-phosphate dehydrogenase, and succinate dehydrogenase were decreased in the corneal epithelium. The activities of lactate dehydrogenase (LDH) and lysosomal hydrolases (LH; acid b-galactosidase, dipeptidyl peptidase II) were increased and appeared in animals immediately after contact lens removal. In rabbits sacrificed 1 h after contact lens removal, LDH and LH activities were increased in the superficial layers of the corneal epithelium. Catalase supplementation during use of contact lenses prevented both the decrease of catalase activity and an addnl. epithelial damage. Topical treatment with 3-aminotriazole resulted a nearly complete loss of catalase activity in the epithelium and a more serious epithelial damage.
TOXICITY OF MONOMERS HEMA, DEGMA AND AEMA, USED TO SYNTHESIS OF HYDROGELS FOR MEDICAL APPLICATIONS
Chemical abstracts: 126:195198
Schneiderka P., Roosova M., Jojkova K., Prokes J., Labsky J., Vacik J. (First Faculty of Med., Charles Univ., Prague, Czech Rep.). Sb. Lek., 97(3), 351-367 (English) 1996 Univerzita Karlova CODEN: SBLEA2. ISSN: 0036-5327. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology)
Acute toxicity parameters (LDs) were evaluated and calcd. after i.m. and i.p. administration of 2-hydroxyethyl methacrylate (HEMA), 2-(hydroxyethoxy)ethyl methacrylate (DEGMA) and 2-(acetoxy)ethyl methacrylate (AEMA) to rats. Our previous findings were revisited and cor. Hematol. parameters revealed low sensitivity to the toxic substances. Clin. chem. tests suggest the prevalence of muscle cell injury.
A NOVEL HYDROPHILIC CROSSLINKER IN PRERPARATION OF HYDROPHILIC SORBENTS
Chemical abstracts: 127:34926
Horak D., Labsky J. (Inst. Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague 162 06, Czech Rep.). React. Funct. Polym., 32(3), 277-280 (English) 1997
A new highly hydrophilic crosslinking agent, 2,3-dihydroxybutane-1,4-diyl dimethacrylate (DHDM), was synthesized from 1,2:3,4-diepoxybutane by reaction with methacrylic acid. Highly hydrophilic spherical particles were prepd. by suspension copolymn. of 2-hydroxyethyl methacrylate (HEMA) with DHDM in an aq. medium in the presence of cyclohexanol and 1-dodecanol as inert diluents. Porous properties of HEMA-DHDM copolymer were compared with those of com. available poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-co-ethylene dimethacrylate) sorbents.
SYNTHESIS AND PROPERTIES OF POWDERY HYDROGELS BASED ON ACRYLAMIDE, METHACRYLAMIDE, AND THEIR N-SUBSTITUTED DERIVATES
Lopour P., Sevcik S., Labsky J., et al.
Angew. Makromol. Chem. 243, 151-159, 1996
