Research topics
Immunoreceptor signalling, lipid rafts, transmembrane adaptor proteins, leukocyte surface molecules
Current Grant Support
Ministry of Education, Youth and Sports of the Czech Republic (Center of Molecular and Cellular Immunology, grant No.: 1M0506; project 2B06064 (NPV2); GACR (project MEM-09-E011).
Most important recent papers
(2004-present; names of the lab members underlined)Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200(8): 1001-13, 2004. (Pubmed) (DOI) (PDF)
Stork B, Engelke M, Frey J, Horejsi V, Hamm-Baarke A, Schraven B, Kurosaki T, Wienands J. Grb2 and the non-T cell activation linker NTAL constitute a Ca(2+)-regulating signal circuit in B lymphocytes. Immunity 21(5): 681-91, 2004. (Pubmed) (DOI) (PDF)
Wang Y, Horvath O, Hamm-Baarke A, Richelme M, Gregoire C, Guinamard R, Horejsi V, Angelisova P, Spicka J, Schraven B, Malissen B, Malissen M. Single and combined deletions of the NTAL/LAB and LAT adaptors minimally affect B-cell development and function. Mol Cell Biol 25(11): 4455-65, 2005. (Pubmed) (DOI)
Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V. Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood 107(1): 213-21, 2006. (Pubmed) (DOI) (PDF)
Tessarz AS, Weiler S, Zanzinger K, Angelisova P, Horejsi V, Cerwenka A. Non-T cell activation linker (NTAL) negatively regulates TREM-1/DAP12-induced inflammatory cytokine production in myeloid cells. J Immunol 178(4): 1991-9, 2007. (Pubmed)
Meraner P, Horejsi V, Wolpl A, Fischer GF, Stingl G, Maurer D. Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol 178(4): 2262-71, 2007. (Pubmed)
Kitaura J, Kawakami Y, Maeda-Yamamoto M, Horejsi V, Kawakami T. Dysregulation of Src family kinases in mast cells from epilepsy-resistant ASK versus epilepsy-prone EL mice. J Immunol 178(1): 455-62, 2007. (Pubmed)
Gregoire C, Simova S, Wang Y, Sansoni A, Richelme S, Schmidt-Giese A, Simeoni L, Angelisova P, Reinhold D, Schraven B, Horejsi V, Malissen B, Malissen M. Deletion of the LIME adaptor protein minimally affects T and B cell development and function. Eur J Immunol 37(11): 3259-69, 2007. (Pubmed) (DOI)
Iwaki S, Spicka J, Tkaczyk C, Jensen BM, Furumoto Y, Charles N, Kovarova M, Rivera J, Horejsi V, Metcalfe DD, Gilfillan AM. Kit- and Fc epsilonRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells. Cell Signal 20(1): 195-205, 2008. (Pubmed) (DOI)
Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermak L, Andera L, Horejsi V, Stockinger H. LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol 45(6): 1703-11, 2008. (Pubmed) (DOI)
Lo Monaco E, Sibilio L, Melucci E, Tremante E, Suchanek M, Horejsi V, Martayan A, Giacomini P. HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides. J Immunol 181(8): 5442-50, 2008. (Pubmed)
Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C. The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol 182(3): 603-14, 2008. (Pubmed) (DOI)
Wu W, Slastad H, de la Rosa Carrillo D, Frey T, Tjonnfjord G, Boretti E, Aasheim HC, Horejsi V, Lund-Johansen F. Antibody array analysis with label-based detection and resolution of protein size. Mol Cell Proteomics 8(2): 245-57, 2009. (Pubmed) (DOI)
Otahal P, Angelisova P, Hrdinka M, Brdicka T, Novak P, Drbal K, Horejsi V. A new type of membrane raft-like microdomains and their possible involvement in TCR signaling. J Immunol 184(7): 3689-96, 2010. (Pubmed) (DOI)
Reviews
Horejsi V. The roles of membrane microdomains (rafts) in T cell activation. Immunol Rev 191: 148-64, 2003. (Pubmed) (PDF)
Horejsi V, Zhang W, Schraven B. Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol 4(8): 603-16, 2004. (Pubmed) (DOI) (PDF)
Horejsi V. Lipid rafts and their roles in T-cell activation. Microbes Infect 7(2): 310-6, 2005. (Pubmed) (DOI) (PDF)
Other results
Useful monoclonal antibodies (link)
Teaching, lectures, public presentations
Immunology courses, Faculty of Science, 3rd Faculty of Medicine, Charles University, Prague; presentation for Czech students (link)
MB150P14B - Immunology - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P78 - Advances in Immunology I - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P78B - Advances in Immunology II - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P15 - Molecular Immunology - Faculty of Science, Charles University, Prague; semestral course in Czech.