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Development and use of DArTFest Array

With the aim to facilitate high-throughput genome profiling and genetic mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five important species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum. The DArTFest array contains 7680 probes derived from methyl-filtered genomic representations.

The potential use of the DArTFest array:

  • Analysis of genetic diversity
  • Genotyping
  • Analysis of genomic constitution of hybrids
  • Genetic and physical mapping
  • Simultaneous marker-assisted selection for multiple traits or specific genome regions

The databasis for DArTFest array is freely available (http://bioinf.scri.ac.uk/germinate_grasses).

Kopecký, D., Bartoš, J., Lukaszewski, A.J., Baird, J.H., Černoch, V., Kölliker, R., Rognli, O.A., Blois, H., Caig, V., Lübberstedt, T., Studer, B., Shaw, B., Doležel, J. and Kilian, A. (2009). Development and mapping of DArT markers within the Festuca – Lolium complex. BMC Genomics 10:473.