Research topics
Immunoregulation, transplantation immunity, stem cells, regulatory T cells, cytokines and other immunoregulatory molecules, gene expression.
Current grant support
Grant Agency of the Czech Republic Nos. 306/08/0640 and 310/08/H077
EU Programme No. GA 222509
Grant Agency of the Ministry of Education No. 1M0506
Grant Agency of the Academy of Science project No. KAN200520804
Most important recent papers
(2004-present; names of the lab members underlined)Holan V, Vitova A, Pindjakova J, Krulova M, Zajicova A, Filipec M. Corneal stromal cells selectively inhibit production of anti-inflammatory cytokines by activated T cells. Clin Exp Immunol 136(2): 200-6, 2004. (Pubmed) (DOI)
Vitova A, Filipec M, Zajicova A, Krulova M, Holan V. Prevention of corneal allograft rejection in a mouse model of high risk recipients. Br J Ophthalmol 88(10): 1338-42, 2004. (Pubmed) (DOI)
Holan V, Pindjakova J, Zajicova A, Krulova M, Zelezna B, Matousek P, Svoboda P. The activity of inducible nitric oxide synthase in rejected skin xenografts is selectively inhibited by a factor produced by grafted cells. Xenotransplantation 12(3): 227-34, 2005. (Pubmed) (DOI)
Pindjakova J, Vitova A, Krulova M, Zajicova A, Filipec M, Holan V. Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production. Transpl Int 18(7): 854-62, 2005. (Pubmed) (DOI)
Holan V, Pindjakova J, Krulova M, Neuwirth A, Fric J, Zajicova A. Production of nitric oxide during graft rejection is regulated by the Th1/Th2 balance, the arginase activity, and L-arginine metabolism. Transplantation 81(12): 1708-15, 2006. (Pubmed) (DOI)
Kubera M, Roman A, Basta-Kaim A, Budziszewska B, Zajicova A, Holan V, Rogoz Z, Skuza G, Leskiewicz M, Regulska M, Jagla G, Nowak W, Lason W. Effect of acute and repeated treatment with mirtazapine on the immunity of noradrenaline transporter knockout C57BL/6J mice. Pharmacol Biochem Behav 85(4): 813-9, 2006. (Pubmed) (DOI)
Tavandzi U, Prochazka R, Usvald D, Hlucilova J, Vitaskova M, Motlik J, Vitova A, Filipec M, Forrester JV, Holan V. A new model of corneal transplantation in the miniature pig: efficacy of immunosuppressive treatment. Transplantation 83(10): 1401-3, 2007. (Pubmed) (DOI)
Pialek J, Vyskocilova M, Bimova B, Havelkova D, Pialkova J, Dufkova P, Bencova V, Dureje L, Albrecht T, Hauffe HC, Macholan M, Munclinger P, Storchova R, Zajicova A, Holan V, Gregorova S, Forejt J. Development of unique house mouse resources suitable for evolutionary studies of speciation. J Hered 99(1): 34-44, 2008. (Pubmed) (DOI)
Fric J, Marek M, Hruskova V, Holan V, Forstova J. Cellular and humoral immune responses to chimeric EGFP-pseudocapsids derived from the mouse polyomavirus after their intranasal administration. Vaccine 26(26): 3242-51, 2008. (Pubmed) (DOI)
Krulova M, Pokorna K, Lencova A, Fric J, Zajicova A, Filipec M, Forrester JV, Holan V. A rapid separation of two distinct populations of mouse corneal epithelial cells with limbal stem cell characteristics by centrifugation on percoll gradient. Invest Ophthalmol Vis Sci 49(9): 3903-8, 2008. (Pubmed) (DOI)
Prochazkova J, Fric J, Pokorna K, Neuwirth A, Krulova M, Zajicova A, Holan V. Distinct regulatory roles of transforming growth factor-beta and interleukin-4 in the development and maintenance of natural and induced CD4+ CD25+ Foxp3+ regulatory T cells. Immunology 128(1 Suppl): e670-8, 2009. (Pubmed) (DOI)
Lipoldova M, Havelkova H, Badalova J, Vojtiskova J, Quan L, Krulova M, Sohrabi Y, Stassen AP, Demant P. Loci controlling lymphocyte production of interferon gamma after alloantigen stimulation in vitro and their co-localization with genes controlling lymphocyte infiltration of tumors and tumor susceptibility. Cancer Immunol Immunother. 2009 Aug 5. [Epub ahead of print] (Pubmed) (DOI)
Furugaki K, Pokorna K, Le Pogam C, Aoki M, Reboul M, Bajzik V, Krief P, Janin A, Noguera ME, West R, Charron D, Chomienne C, Pla M, Moins-Teisserenc H, Padua RA. DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model. Blood 115(3): 653-6, 2010. (Pubmed) (DOI)
Holan V, Pokorna K, Prochazkova J, Krulova M, Zajicova A. Immunoregulatory properties of mouse limbal stem cells. J Immunol 184(4): 2124-9, 2010. (Pubmed) (DOI)
Reviews
Holan V. Corneal stromal cells selectively inhibit the production of certain anti-inflammatory cytokines. Expert Rev. Clin. Immunol. 2: 101-8, 2006.
Teaching, lectures, public presentations
MB150P13 - Regulatory Mechanisms of Immunity - Faculty of Science, Charles University, Prague; semestral course in Czech.