Research topics

Modulation of microtubule properties by signal transduction molecules, function of gamma-tubulin forms, microtubule-associated proteins, interactions between microtubules and other cellular components. Tubulins as markers of tumor cells.

Current grant support

Ministry of Education, Youth and Sports of the Czech Republic (Center of Cell Functional Organization, grant No.: LC545)
Grant Agency of the Czech Republic (grants Nos.: 204/09/1777, 204/09/H084, P302/10/1701)
Grant Agency of the Academy of Sciences of the Czech Republic (KAN200520701)

Most important recent papers

(2004-present; names of the lab members underlined)

Libusova L, Sulimenko T, Sulimenko V, Hozak P, Draber P. gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms. Exp Cell Res 295(2): 375-86, 2004. (Pubmed) (DOI)

Sulimenko T, Draber P. A fast and simple dot-immunobinding assay for quantification of mouse immunoglobulins in hybridoma culture supernatants. J Immunol Methods 289(1-2): 89-95, 2004. (Pubmed) (DOI)

Kukharskyy V, Sulimenko V, Macurek L, Sulimenko T, Draberova E, Draber P. Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res 298(1): 218-28, 2004. (Pubmed) (DOI)

Katsetos CD, Reddy G, Draberova E, Smejkalova B, Del Valle L, Ashraf Q, Tadevosyan A, Yelin K, Maraziotis T, Mishra OP, Mork S, Legido A, Nissanov J, Baas PW, de Chadarevian JP, Draber P. Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines. J Neuropathol Exp Neurol 65(5): 465-77, 2006. (Pubmed) (DOI)

Sulimenko V, Draberova E, Sulimenko T, Macurek L, Richterova V, Draber P, Draber P. Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol 176(12): 7243-53, 2006. (Pubmed)

Peknicova J, Pexidrova M, Kubatova A, Koubek P, Tepla O, Sulimenko T, Draber P. Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil Steril 88(4 Suppl): 1120-8, 2007. (Pubmed) (DOI)

Katsetos CD, Draberova E, Smejkalova B, Reddy G, Bertrand L, de Chadarevian JP, Legido A, Nissanov J, Baas PW, Draber P. Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells. Neurochem Res 32(8): 1387-98, 2007. (Pubmed) (DOI)

Draberova E, Del Valle L, Gordon J, Markova V, Smejkalova B, Bertrand L, de Chadarevian JP, Agamanolis DP, Legido A, Khalili K, Draber P, Katsetos CD. Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity. J Neuropathol Exp Neurol 67(4): 341-54, 2008. (Pubmed) (DOI)

Blume Y, Yemets A, Sulimenko V, Sulimenko T, Chan J, Lloyd C, Draber P. Tyrosine phosphorylation of plant tubulin. Planta 229(1): 143-50, 2008. (Pubmed) (DOI)

Macurek L, Draberova E, Richterova V, Sulimenko V, Sulimenko T, Draberova L, Markova V, Draber P. Regulation of microtubule nucleation from membranes by complexes of membrane-bound gamma-tubulin with Fyn kinase and phosphoinositide 3-kinase. Biochem J 416(3): 421-30, 2008. (Pubmed) (DOI)

Draberova E, Sulimenko V, Sulimenko T, Bohm KJ, Draber P. Recovery of tubulin functions after freeze-drying in the presence of trehalose. Anal Biochem 397(1): 67-72, 2010. (Pubmed) (DOI)

Blume Y, Yemets A, Sheremet Y, Nyporko A, Sulimenko V, Sulimenko T, Draber P. Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol 10(1): 29, 2010. (Pubmed) (DOI)

Caracciolo V, D'agostino L, Draberova E, Sladkova V, Crozier-Fitzgerald C, Agamanolis DP, de Chadarevian JP, Legido A, Giordano A, Draber P, Katsetos CD. Differential expression and cellular distribution of gamma-tubulin and betaIII-tubulin in medulloblastomas and human medulloblastoma cell lines. J Cell Physiol 223(2): 519-29, 2010. (Pubmed) (DOI)

Hajkova Z, Bugajev V, Draberova E, Vinopal S, Draberova L, Janacek J, Draber P, Draber P. STIM1-directed reorganization of microtubules in activated mast cells. J Immunol 186(2): 913-23, 2011. (Pubmed) (DOI)

Reviews

Binarova P, Cenklova V, Pochylova Z, Draberova E, Draber P. Gamma-tubulins and their functions in plant cells. In: The Plant Cytoskeleton. Eds: YB Blume, VW Baird, AI Yemets, D Breviario. Springer Verlag, ISBN: 987-1-4020-8841-4, pp 23-43, 2008.

Katsetos CD, Draberova E, Legido A, Dumontet C, Draber P. Tubulin targets in the pathobiology and therapy of glioblastoma multiforme. I. Class III beta-tubulin. J Cell Physiol 221(3): 505-13, 2009. (Pubmed) (DOI)

Katsetos CD, Draberova E, Legido A, Draber P. Tubulin targets in the pathobiology and therapy of glioblastoma multiforme. II. gamma-Tubulin. J Cell Physiol 221(3): 514-20, 2009. (Pubmed) (DOI)

Other results

Monoclonal antibodies against cytoskeletal proteins (link) + (Exbio).

Teaching, lectures, public presentations