Research topics

Mast cells activation, transmembrane signaling, functional networks and topography of plasma membrane components, plasma membrane microdomains, endocytosis and exocytosis.

Current grant support

Ministry of Education, Youth and Sports of the Czech Republic (Center of Molecular and Cellular Immunology, grant No.: 1M0506)
Grant Agency of the Czech Republic (grant No.: 301/09/1826 and P302/10/1759)
Academy of Sciences of the Czech Republic [grant No.: KAN200520701 (Program Nanotechnology for society) and M200520901 (Program supporting international colaboration)

Most important recent papers

(2004-present; names of the lab members underlined)

Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM. NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood 104(1): 207-14, 2004. (Pubmed) (DOI)

Volna P, Lebduska P, Draberova L, Simova S, Heneberg P, Boubelik M, Bugajev V, Malissen B, Wilson BS, Horejsi V, Malissen M, Draber P. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200(8): 1001-13, 2004. (Pubmed) (DOI)

Tolar P, Draberova L, Tolarova H, Draber P. Positive and negative regulation of Fc epsilon receptor I-mediated signaling events by Lyn kinase C-terminal tyrosine phosphorylation. Eur J Immunol 34(4): 1136-45, 2004. (Pubmed) (DOI)

Tolarova H, Draberova L, Heneberg P, Draber P. Involvement of filamentous actin in setting the threshold for degranulation in mast cells. Eur J Immunol 34(6): 1627-36, 2004. (Pubmed) (DOI)

Draberova L, Lebduska P, Halova I, Tolar P, Stokrova J, Tolarova H, Korb J, Draber P. Signaling assemblies formed in mast cells activated via Fcepsilon receptor I dimers. Eur J Immunol 34(8): 2209-19, 2004. (Pubmed) (DOI)

Sulimenko V, Draberova E, Sulimenko T, Macurek L, Richterova V, Draber P, Draber P. Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol 176(12): 7243-53, 2006. (Pubmed)

Heneberg P, Lebduska P, Draberova L, Korb J, Draber P. Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol 36(10): 2795-806, 2006. (Pubmed) (DOI)

Smrz D, Draberova L, Draber P. Non-apoptotic phosphatidylserine externalization induced by engagement of glycosylphosphatidylinositol-anchored proteins. J Biol Chem 282(14): 10487-97, 2007. (Pubmed) (DOI)

Draberova L, Shaik GM, Volna P, Heneberg P, Tumova M, Lebduska P, Korb J, Draber P. Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker. J Immunol 179(8): 5169-80, 2007. (Pubmed)

Draber P, Draberova L, Heneberg P, Smid F, Farghali H, Draber P. Preformed STAT3 transducer complexes in human HepG2 cells and rat hepatocytes. Cell Signal 19(11): 2400-12, 2007. (Pubmed) (DOI)

Lebduska P, Korb J, Tumova M, Heneberg P, Draber P. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods 328(1-2): 139-51, 2007. (Pubmed) (DOI)

Jirkovska M, Majer F, Smidova J, Stritesky J, Shaik GM, Draber P, Vitek L, Marecek Z, Smid F. Changes in GM1 ganglioside content and localization in cholestatic rat liver. Glycoconj J 24(4-5): 231-41, 2007. (Pubmed) (DOI)

Shaik GM, Draberova L, Draber P, Boubelik M, Draber P. Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I. Nucleic Acids Res 36(15): e93, 2008. (Pubmed) (DOI)

Smrz D, Lebduska P, Draberova L, Korb J, Draber P. Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem 283(16): 10904-18, 2008. (Pubmed) (DOI)

Shaik GM, Draberova L, Heneberg P, Draber P. Vacuolin-1-modulated exocytosis and cell resealing in mast cells. Cell Signal 21(8): 1337-45, 2009. (Pubmed) (DOI)

Heneberg P, Draberova L, Bambouskova M, Pompach P, Draber P. Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts. J Biol Chem 285(17): 12787-802, 2010. (Pubmed) (DOI)

Tumova M, Koffer A, Simicek M, Draberova L, Draber P. The transmembrane adaptor protein NTAL signals to mast cell cytoskeleton via the small GTPase Rho. Eur J Immunol 40(11): 3235-45, 2010. (Pubmed) (DOI)

Bugajev V, Bambouskova M, Draberova L, Draber P. What precedes the initial tyrosine phosphorylation of the high affinity IgE receptor in antigen-activated mast cell? FEBS Lett 584(24): 4949-55, 2010. (Pubmed) (DOI)

Hajkova Z, Bugajev V, Draberova E, Vinopal S, Draberova L, Janacek J, Draber P, Draber P. STIM1-directed reorganization of microtubules in activated mast cells. J Immunol 186(2): 913-23, 2011. (Pubmed) (DOI)

Reviews

Heneberg P, Draber P. Nonreceptor protein tyrosine and lipid phosphatases in type I fc(epsilon) receptor-mediated activation of mast cells and basophils. Int Arch Allergy Immunol 128(4): 253-63, 2002. (Pubmed)

Luskova P, Draber P. Modulation of the Fcepsilon receptor I signaling by tyrosine kinase inhibitors: search for therapeutic targets of inflammatory and allergy diseases. Curr Pharm Des 10(15): 1727-37, 2004. (Pubmed)

Heneberg P, Draber P. Regulation of cys-based protein tyrosine phosphatases via reactive oxygen and nitrogen species in mast cells and basophils. Curr Med Chem 12(16): 1859-71, 2005. (Pubmed)

Draber P, Draberova L. Lifting the fog in store-operated Ca2+ entry. Trends Immunol 26(12): 621-4, 2005. (Pubmed) (DOI)

Other results

Monoclonal antibodies for commercial purposes (www.exbio.cz, www.top-bio.cz).
New solution useful for polymerase chain reaction (www.top-bio.cz).

Teaching, lectures, public presentations