Research topics
Immunoreceptor signalling, lipid rafts, transmembrane adaptor proteins, leukocyte surface molecules
Current Grant Support
Ministry of Education, Youth and Sports of the Czech Republic (Center of Molecular and Cellular Immunology, grant No.: 1M0506; GACR (project MEM-09-E011).
Most important recent papers
(2006-present; names of the lab members underlined)Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V. Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood 107(1): 213-21, 2006. (Pubmed) (DOI) (PDF)
Tessarz AS, Weiler S, Zanzinger K, Angelisova P, Horejsi V, Cerwenka A. Non-T cell activation linker (NTAL) negatively regulates TREM-1/DAP12-induced inflammatory cytokine production in myeloid cells. J Immunol 178(4): 1991-9, 2007. (Pubmed)
Meraner P, Horejsi V, Wolpl A, Fischer GF, Stingl G, Maurer D. Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol 178(4): 2262-71, 2007. (Pubmed)
Kitaura J, Kawakami Y, Maeda-Yamamoto M, Horejsi V, Kawakami T. Dysregulation of Src family kinases in mast cells from epilepsy-resistant ASK versus epilepsy-prone EL mice. J Immunol 178(1): 455-62, 2007. (Pubmed)
Gregoire C, Simova S, Wang Y, Sansoni A, Richelme S, Schmidt-Giese A, Simeoni L, Angelisova P, Reinhold D, Schraven B, Horejsi V, Malissen B, Malissen M. Deletion of the LIME adaptor protein minimally affects T and B cell development and function. Eur J Immunol 37(11): 3259-69, 2007. (Pubmed) (DOI)
Iwaki S, Spicka J, Tkaczyk C, Jensen BM, Furumoto Y, Charles N, Kovarova M, Rivera J, Horejsi V, Metcalfe DD, Gilfillan AM. Kit- and Fc epsilonRI-induced differential phosphorylation of the transmembrane adaptor molecule NTAL/LAB/LAT2 allows flexibility in its scaffolding function in mast cells. Cell Signal 20(1): 195-205, 2008. (Pubmed) (DOI)
Lo Monaco E, Sibilio L, Melucci E, Tremante E, Suchanek M, Horejsi V, Martayan A, Giacomini P. HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides. J Immunol 181(8): 5442-50, 2008. (Pubmed)
Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C. The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1. J Cell Biol 182(3): 603-14, 2008. (Pubmed) (DOI)
Wu W, Slastad H, de la Rosa Carrillo D, Frey T, Tjonnfjord G, Boretti E, Aasheim HC, Horejsi V, Lund-Johansen F. Antibody array analysis with label-based detection and resolution of protein size. Mol Cell Proteomics 8(2): 245-57, 2009. (Pubmed) (DOI)
Otahal P, Angelisova P, Hrdinka M, Brdicka T, Novak P, Drbal K, Horejsi V. A new type of membrane raft-like microdomains and their possible involvement in TCR signaling. J Immunol 184(7): 3689-96, 2010. (Pubmed) (DOI)
Otahal P, Pata S, Angelisova P, Horejsi V, Brdicka T. The effects of membrane compartmentalization of csk on TCR signaling. Biochim Biophys Acta 1813(2): 367-76, 2011. (Pubmed) (DOI)
Hrdinka M, Draber P, Stepanek O, Ormsby T, Otahal P, Angelisova P, Brdicka T, Paces J, Horejsi V, Drbal K. PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J Biol Chem 286(22): 19617-29, 2011. (Pubmed) (DOI)
Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T. Regulation of Src Family Kinases Involved in T Cell Receptor Signaling by Protein-tyrosine Phosphatase CD148. J Biol Chem 286(25): 22101-12, 2011. (Pubmed) (DOI)
Stepanek O, Brdicka T, Angelisova P, Horvath O, Spicka J, Stockbauer P, Man P, Horejsi V. Interaction of late apoptotic and necrotic cells with vitronectin. PLoS One 6(5): e19243, 2011. (Pubmed) (DOI)
Ormsby T, Schlecker E, Ferdin J, Tessarz AS, Angelisova P, Koprulu AD, Borte M, Warnatz K, Schulze I, Ellmeier W, Horejsi V, Cerwenka A. Btk is a positive regulator in the TREM-1/DAP12 signaling pathway. Blood 118(4): 936-45, 2011. (Pubmed) (DOI)
Reviews
Horejsi V, Zhang W, Schraven B. Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol 4(8): 603-16, 2004. (Pubmed) (DOI) (PDF)
Horejsi V. Lipid rafts and their roles in T-cell activation. Microbes Infect 7(2): 310-6, 2005. (Pubmed) (DOI) (PDF)
Horejsi V, Otahal P, Brdicka T. LAT--an important raft-associated transmembrane adaptor protein. Delivered on 6 July 2009 at the 34th FEBS Congress in Prague, Czech Republic. FEBS J 277(21): 4383-97, 2010. (Pubmed) (DOI)
Other results
Useful monoclonal antibodies (link)
Teaching, lectures, public presentations
Immunology courses, Faculty of Science, 3rd Faculty of Medicine, Charles University, Prague; presentation for Czech students (link)
MB150P14B - Immunology - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P78 - Advances in Immunology I - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P78B - Advances in Immunology II - Faculty of Science, Charles University, Prague; semestral course in Czech.
MB150P15 - Molecular Immunology - Faculty of Science, Charles University, Prague; semestral course in Czech.