Main Topics:
1. Structure and function of purinergic P2X receptors.
2. Purinergic signaling in pituitary and hypothalamus.
3. Melatonin receptors and excitability of neonatal pituitary
gonadotrophs.
Research:
Laboratory is focussed on the research of pituitary and hypothalamus.
The main interest is on functional expression of purinergic P2X
receptors and melatonin receptors that control intracellular calcium
signaling, excitability and secretion of hormones from anterior
pituitary. Using perforated patch clamp recording and microfluorimetric
techniques, the effect of melatonin on GnRH-stimulated electrical
activity and Ca2+ oscillations is studied in primary culture of neonatal
pituitary cells. In neurons of suprachiasmatic nuclei (SCN) and
supraoptic nuclei (SON) in hypothalamic rat brain slices, expression,
physiological and pharmacological properties of purinergic P2X receptors
is investigated. Organotypic SCN slices are used to monitor circadian
rhythm of ATP release. Recent research is focused on structure-function
relationship of recombinant purinergic P2X receptors and melatonin
receptors studied using mutagenesis and expression of mutated receptors
in transfected HEK293 cells.
PUBLICATIONS:
(Authors of the Department are underlined.)
2009
Balík A, Jindrichová M, Bhattacharyya S, Zemková H: GnRH-I and
GnRH-II-induced calcium signaling and hormone secretion in neonatal rat
gonadotrophs. Phys Res 58 (5), 2009 (in press).
Jindrichova M, Vavra V, Obsil T, Stojilkovic SS, Zemkova H:
Functional relevance of aromatic residues in the first transmembrane
domain of P2X receptors. J Neurochem, 2009 (in press)
2008
Jelínková I, Vávra V, Jindrichová M, Obsil T, Zemková HW,
Zemková H, Stojilkovic SS: Identification of P2X4 Receptor
Transmembrane Residues Contributing to Channel Gating and Interaction
with Ivermectin. Pflugers Arch, 456: 939-950, 2008.
Zemkova WH, Bjelobaba I, Tomic M, Zemkova H, Stojilkovic SS:
Characterization of Depolarizing GABAA Receptor-Channels in Anterior
Pituitary Cells. J Physiol 586: 3097–3111, 2008
Mazna P, Grycova L, Balik A, Zemkova H, Friedlova E, Obsilova V,
Obsil T, Teisinger J:The role of proline residues in the structure and
function of human MT2 melatonin receptor. J Pineal Res. 45:361-372. 2008
.
Zemková H, Balík A, Jindrichová M, Vávra V: Molecular Structure
of Purinergic P2X Receptors and their Expression in the Hypothalamus and
Pituitary. Physiol Res. 2008 May 13. Phys Res 57: S23-S38, 2008.
Zemkova H, Balik A, Mazna P: Melatonin Inhibition of
Gonadotropin-Releasing Hormone-Induced Calcium Signaling and Hormone
Secretion in Neonatal Pituitary Gonadotrophs. Chapter 5, pp.59-82. In:
Haldar C, Singaravel M, Pandi-Perumal SR, Cardinali DP eds. Experimental
Endocrinology and Reproductive Biology. Georgetown, TX, ISBN
978-1-57808-518-7; February 2008; 335 pages, © 2008 LANDES BIOSCIENCE
2007
Zemkova H, Yan Z, Liang Z, Jelinkova I, Tomic M.,
Stojilkovic SS (2007) Role of Aromatic and Charged Ectodomain Residues
in the P2X4 Receptor Functions. J Neurochem 102:1139-1150, 2007.
2006
Zemkova H, Balik A, Jiang Y, Kretschmannova K, Stojilkovic SS
(2006) Roles of purinergic P2X receptors as pacemaking channels and
modulators of calcium-mobilizing pathway in pituitary gonadotrophs. Mol
Endocrinol 20:1423-1436.
Gonzalez-Iglesias AE, Jiang Y, Tomic M, Kretschmannova K, Andric
SA, Zemkova H, Stojilkovic SS (2006) Dependence of electrical
activity and calcium influx-controlled prolactin release on adenylyl
cyclase signaling pathway in pituitary lactotrophs. Mol Endocrinol
20:2231-2246.
Jelinkova I, Yan Z, Liang Z, Moonat S, Teisinger J,
Stojilkovic SS, Zemkova H (2006) Identification of P2X(4)
receptor-specific residues contributing to the ivermectin effects on
channel deactivation. Biochem Biophys Res Commun 349:619-625.
Kretschmannova K, Gonzalez-Iglesias AE, Tomic M, Stojilkovic SS
(2006) Dependence of hyperpolarisation-activated cyclic nucleotide-gated
channel activity on basal cyclic adenosine monophosphate production in
spontaneously firing GH3 cells. J Neuroendocrinol 18:484-493.
Koshimizu TA, Kretschmannova K, He ML, Ueno S, Tanoue A,
Yanagihara N, Stojilkovic SS, Tsujimoto G (2006) Carboxyl-terminal
splicing enhances physical interactions between the cytoplasmic tails of
purinergic P2X receptors. Mol Pharmacol 69:1588-1598.
Gonzalez-Iglesias AE, Kretschmannova K, Tomic M, Stojilkovic SS
(2006) ZD7288 inhibits exocytosis in an HCN-independent manner and
downstream of voltage-gated calcium influx in pituitary lactotrophs.
Biochem Biophys Res Commun 346:845-850.
2005
Stojilkovic SS, Tomic M, He ML, Yan Z, Koshimizu T, Zemkova H
(2005) Molecular Dissection of Purinergic P2X Receptor-Channels. Ann N Y
Acad Sci 1048, 116-130.
Kretschmannova K, Svobodova I, Balik A, Mazna P & Zemkova H
(2005) Circadian Rhythmicity in AVP Secretion and GABAergic Synaptic
Transmission in the Rat Suprachiasmatic Nucleus. Ann N Y Acad Sci 1048,
103-15.
Mazna P, Berka K, Jelinkova I, Balik A, Svoboda P, Obsilova V,
Obsil T, Teisinger J (2005) Ligand binding to the human MT2 melatonin
receptor: the role of residues in transmembrane domains 3, 6, and 7.
Biochem Biophys Res Commun 332, 726-34.
Stojilkovic SS, Zemkova H & Van Goor F (2005) Biophysical basis
of pituitary cell type-specific Ca2+ signaling-secretion coupling.
Trends Endocrinol Metab 16, 152-9.
Zemková H, Balík A, Stojilkovic SS (2005) Expression and Signal
Transduction Pathways of Melatonin Receptors in Pituitary. Chapter 8,
In: Pandi-Perumal SR, Cardinali DP eds. Melatonin: Biological Basis
of its Function in Health and Disease. Georgetowen, TX Landis
Bioscience, 1-19,
http://www.eurekah.com
2004
Balik A, Kretschmannova K, Mazna P, Svobodova I & Zemkova H
(2004) Melatonin action in neonatal gonadotrophs. Physiol Res 53 Suppl
1, S153-66.
Kretschmannova K & Zemkova H (2004) Characterization of
neuromuscular transmission in mice with progressive motoneuronopathy.
Physiol Res 53, 541-8.
Mazna P, Obsilova V, Jelinkova I, Balik A, Berka K, Sovova Z,
Ettrich R, Svoboda P, Obsil T & Teisinger J (2004) Molecular modeling of
human MT2 melatonin receptor: the role of Val204, Leu272 and Tyr298 in
ligand binding. J Neurochem 91, 836-42.
Wise PM, Radil T & Wysocki CJ (2004) Temporal integration in
nasal lateralization and nasal detection of carbon dioxide. Chem Senses
29, 137-42.
Zemkova H, Balik A, Kretschmannova K, Mazna P & Stojilkovic SS
(2004) Recovery of Ins(1,4,5)-trisphosphate-dependent calcium signaling
in neonatal gonadotrophs. Cell Calcium 36, 89-97.
Zemkova H, He ML, Koshimizu TA & Stojilkovic SS (2004)
Identification of ectodomain regions contributing to gating,
deactivation, and resensitization of purinergic P2X receptors. J
Neurosci 24, 6968-78.
2003
He ML, Zemkova H, Koshimizu TA, Tomic M & Stojilkovic SS (2003)
Intracellular calcium measurements as a method in studies on activity of
purinergic P2X receptor channels. Am J Physiol Cell Physiol 285,
C467-79.
He ML, Zemkova H & Stojilkovic SS (2003) Dependence of purinergic
P2X receptor activity on ectodomain structure. J Biol Chem 278, 10182-8.
Kretschmannova K, Svobodova I & Zemkova H (2003) Day-night
variations in zinc sensitivity of GABAA receptor-channels in rat
suprachiasmatic nucleus. Brain Res Mol Brain Res 120, 46-51.
Svobodova I, Vanecek J & Zemkova H (2003) The bidirectional phase-shifting
effects of melatonin on the arginine vasopressin secretion rhythm in rat
suprachiasmatic nuclei in vitro. Brain Res Mol Brain Res 116, 80-5.
Wise PM, Wysocki CJ & Radil T (2003) Time-intensity ratings of
nasal irritation from carbon dioxide. Chem Senses 28, 751-60.
Wysocki CJ, Cowart BJ & Radil T (2003) Nasal trigeminal
chemosensitivity across the adult life span. Percept Psychophys 65,
115-22 .
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