Research topics
DNA damage response; DNA repair mechanisms; hereditary DNA repair disorders.
Current Grant Support
Grant Agency of the Czech Republic (2009-2013; grant No. GA204/09/0565): Role of RECQ5 DNA helicase in maintenance of genomic stability.
Grant Agency of the Czech Republic (2010-2014; grant No.P305/10/0281): Role of the Rothmund-Thomson syndrome gene product in maintenance of genomic stability.
Most important recent papers
(2008-present; names of the lab members underlined)Selak N, Bachrati CZ, Shevelev I, Dietschy T, van Loon B, Jacob A, Hubscher U, Hoheisel JD, Hickson ID, Stagljar I. The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta. Nucleic Acids Res 36(16): 5166-79, 2008. (Pubmed) (DOI)
Dietschy T, Shevelev I, Pena-Diaz J, Huhn D, Kuenzle S, Mak R, Miah MF, Hess D, Fey M, Hottiger MO, Janscak P, Stagljar I. p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization. J Cell Sci 122(Pt 8): 1258-67, 2009. (Pubmed) (DOI)
Zheng L, Kanagaraj R, Mihaljevic B, Schwendener S, Sartori AA, Gerrits B, Shevelev I, Janscak P. MRE11 complex links RECQ5 helicase to sites of DNA damage. Nucleic Acids Res 37(8): 2645-57, 2009. (Pubmed) (DOI)
Yodh JG, Stevens BC, Kanagaraj R, Janscak P, Ha T. BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation. EMBO J 28(4): 405-16, 2009. (Pubmed) (DOI)
Schwendener S, Raynard S, Paliwal S, Cheng A, Kanagaraj R, Shevelev I, Stark JM, Sung P, Janscak P. Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity. J Biol Chem 285(21): 15739-45, 2010. (Pubmed) (DOI)
Kanagaraj R, Huehn D, MacKellar A, Menigatti M, Zheng L, Urban V, Shevelev I, Greenleaf AL, Janscak P. RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription. Nucleic Acids Res 38(22): 8131-40, 2010. (Pubmed) (DOI)
Reviews
Shevelev IV, Hubscher U. The 3' 5' exonucleases. Nat Rev Mol Cell Biol 3(5): 364-76, 2002. (Pubmed) (DOI)
Ramadan K, Shevelev I, Hubscher U. The DNA-polymerase-X family: controllers of DNA quality? Nat Rev Mol Cell Biol 5(12): 1038-43, 2004. (Pubmed) (DOI)
Dietschy T, Shevelev I, Stagljar I. The molecular role of the Rothmund-Thomson-, RAPADILINO- and Baller-Gerold-gene product, RECQL4: recent progress. Cell Mol Life Sci 64(7-8): 796-802, 2007. (Pubmed) (DOI)