Research topics
Epithelial and epidermal compartments, liver, inflammation, experimental colatis and IBD, matrix metalloproteinases and ADAMs, kallikreins and their inhibitors, adhesion molecules, extracellular matrix proteins, cell-matrix interactions.
Current Grant Support
OPVK, MŠMT (from June 2011), Founding of an expert team for the Centre for Phenogenomics.
GACR (P302/11/2048, from January 2011), Function of metalloproteinases in colon epithelium and during development of experimental colitis and colon cancer.
GACR (P305/10/2143, recent grant), Generation of mouse models for targeting stellate cells and myofibroblasts in the liver.
GACR (P303/10/2044, recent grant, Jirouskova/Sedlacek), The Impact of a Liver-Specific Deficiency of Growth Factor Sheddase ADAM10 on Liver Development and Pathology.
GAAV CR IAA500520812, Investigating the pathologic role of metalloproteinases in liver.
Most important recent papers
(2004-present; names of the lab members underlined)Jirouskova M, Chereshnev I, Vaananen H, Degen JL, Coller BS. Antibody blockade or mutation of the fibrinogen gamma-chain C-terminus is more effective in inhibiting murine arterial thrombus formation than complete absence of fibrinogen. Blood 103(6): 1995-2002, 2004. (Pubmed) (DOI)
Abel S, Hundhausen C, Mentlein R, Schulte A, Berkhout TA, Broadway N, Hartmann D, Sedlacek R, Dietrich S, Muetze B, Schuster B, Kallen KJ, Saftig P, Rose-John S, Ludwig A. The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin-like metalloproteinase ADAM10. J Immunol 172(10): 6362-72, 2004. (Pubmed)
Flick MJ, Du X, Witte DP, Jirouskova M, Soloviev DA, Busuttil SJ, Plow EF, Degen JL. Leukocyte engagement of fibrin(ogen) via the integrin receptor alphaMbeta2/Mac-1 is critical for host inflammatory response in vivo. J Clin Invest 113(11): 1596-606, 2004. (Pubmed) (DOI)
Titz B, Dietrich S, Sadowski T, Beck C, Petersen A, Sedlacek R. Activity of MMP-19 inhibits capillary-like formation due to processing of nidogen-1. Cell Mol Life Sci 61(14): 1826-33, 2004. (Pubmed) (DOI)
Palumbo JS, Talmage KE, Massari JV, La Jeunesse CM, Flick MJ, Kombrinck KW, Jirouskova M, Degen JL. Platelets and fibrin(ogen) increase metastatic potential by impeding natural killer cell-mediated elimination of tumor cells. Blood 105(1): 178-85, 2005. (Pubmed) (DOI)
Sadowski T, Dietrich S, Koschinsky F, Ludwig A, Proksch E, Titz B, Sedlacek R. Matrix metalloproteinase 19 processes the laminin 5 gamma 2 chain and induces epithelial cell migration. Cell Mol Life Sci 62(7-8): 870-80, 2005. (Pubmed) (DOI)
Beck IM, Muller M, Mentlein R, Sadowski T, Mueller MS, Paus R, Sedlacek R. Matrix metalloproteinase-19 expression in keratinocytes is repressed by transcription factors Tst-1 and Skn-1a: implications for keratinocyte differentiation. J Invest Dermatol 127(5): 1107-14, 2007. (Pubmed) (DOI)
Jirouskova M, Jaiswal JK, Coller BS. Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading. Blood 109(12): 5260-9, 2007. (Pubmed) (DOI)
Beck IM, Ruckert R, Brandt K, Mueller MS, Sadowski T, Brauer R, Schirmacher P, Mentlein R, Sedlacek R. MMP19 is essential for T cell development and T cell-mediated cutaneous immune responses. PLoS One 3(6): e2343, 2008. (Pubmed) (DOI)
Lettau I, Hattermann K, Held-Feindt J, Brauer R, Sedlacek R, Mentlein R. Matrix metalloproteinase-19 is highly expressed in astroglial tumors and promotes invasion of glioma cells. J Neuropathol Exp Neurol 69(3): 215-23, 2010. (Pubmed) (DOI)
Muller M, Beck IM, Gadesmann J, Karschuk N, Paschen A, Proksch E, Djonov V, Reiss K, Sedlacek R. MMP19 is upregulated during melanoma progression and increases invasion of melanoma cells. Mod Pathol. 2010 Jan 22. [Epub ahead of print] (Pubmed) (DOI)
Chan KC, Ko JM, Lung HL, Sedlacek R, Zhang ZF, Luo DZ, Feng ZB, Chen S, Chen H, Chan KW, Tsao SW, Chua DT, Zabarovsky ER, Stanbridge EJ, Lung ML. Catalytic activity of matrix metalloproteinase-19 is essential for tumor suppressor and anti-angiogenic activities in nasopharyngeal carcinoma. Int J Cancer. 2010 Dec 16. [Epub ahead of print] (Pubmed) (DOI)
Reviews
Emeis JJ, Jirouskova M, Muchitsch EM, Shet AS, Smyth SS, Johnson GJ. A guide to murine coagulation factor structure, function, assays, and genetic alterations. J Thromb Haemost 5(4): 670-9, 2007. (Pubmed) (DOI)
Jirouskova M, Shet AS, Johnson GJ. A guide to murine platelet structure, function, assays, and genetic alterations. J Thromb Haemost 5(4): 661-9, 2007. (Pubmed) (DOI)
Other results
Department is offering embryo manipulating services including DNA pronuclear microinjections, generation of mutant mice using ES cell technology, sperm freezing and archiving, check of sperm quality, in vitro fertilization (IVF), embryo isolations, rederivation of mouse lines/strains, vasectomy, etc.