1. Cellular and molecular mechanisms of desensitisation of hormone response. Agonist-induced subcellular redistribution of GPCR (G protein coupled receptors), agonist-induced subcellular redistribution of trimeric G proteins, soluble (cytosolic) forms of trimeric G proteins, detergent-resistant membrane domains (DRMs), hormone-induced changes in composition of DRMs, functional activity of trimeric G-proteins in plasma membrane compartments. This work is performed in selected cell lines stably expressing the specific types of GPCR, their cognate G proteins or the fusion proteins between GPCR and GFP (green fluorescent protein); in support from Glasgow University, Scotland, UK (Prof. Graeme Milligan).

2. GABAB-R initiated signalling cascade in the brain; studies of isolated plasma membrane fractions, ontogenetic development; distribution in different brain areas.

3. Beta-adrenergic signalling cascade in the heart muscle; changes induced by the long term agonist stimulation; hydrophobic membrane interactions.

Publications