Hlavní oblasti výzkumu:
 
1. Struktůra a funkce purinergních P2X receptorů.
2. Purinergní signalizace v hypothalamu.
3. Melatoninové receptory a excitabilita neonatálních gonádotropních buněk hypofýzy.

Laboratoř se věnuje výzkumu hypofýzy a hypothalamu. Hlavní zájem je soustředěn na funkční expresi purinergních P2X receptorů a melatoninových receptorů, které kontrolují intracelulární vápníkovou signalizaci a sekreci hormonů v adenohypofýze. Elektrofyziologickými, mikrofluorimetrickými a radioimunologickými technikami je studován vliv melatoninu na GnRH-stimulovanou elekrickou aktivitu, intracelulární Ca2+ signalizaci a LH sekreci v primární kultuře neonatálních hypofyzárních buněk. V neuronech suprachiasmatického jádra (SCN) a supraoptického jádra (SON) v akutně izolovanýách krysích mozkových řezech je studována modulace synaptického přenosu purinergními P2X receptory. Výzkum v poslední době je zaměřen na vztah mezi strukturou a funkcí rekombinantních purinergních P2X receptorů, které jsou studovány pomocí bodových mutací a po expresi mutovaných receptorů v HEK293 buňkách.

PUBLIKACE:
(Jména autorů z laboratoře jsou podtržena)
 
2011
Jindrichova M, Khafizov K, Skorinkin A, Fayuk D, Bart G, Zemkova H, Giniatullin R: Highly conserved tyrosine 37 stabilizes desensitized states and restricts calcium permeability of ATP-gated P2X3 receptor. J Neurochem 119:676-685, 2011.
Petrenko N, Khafizov K, Tvrdonova V, Skorinkin A, Giniatullin R: Role of the ectodomain serine 275 in shaping the binding pocket of the ATP-gated P2X3 receptor. Biochemistry 50:8427-8436, 2011.
Vavra V, Bhattacharya A, Zemkova H: Facilitation of glutamate and GABA release by P2X receptor activation in supraoptic neurons from freshly isolated rat brain slices. Neuroscience 188:1-12, 2011.
Zemkova H, Stojilkovic SS, Klein DC: Norepinephrine causes a biphasic change in mammalian pinealocye membrane potential: role of alpha1B-adrenoreceptors, phospholipase C, and Ca2+. Endocrinology 152:3842-3851, 2011.

2010
Stojilkovic SS, He ML, Koshimizu TA, Balik A, Zemkova H: Signaling by purinergic receptors and channels in the pituitary gland. Mol Cell Endocrinol. 314: 184-191, 2010.
Kucka M, Kretschmannova K, Murano T, Wu CP, Zemkova H, Ambudkar SV, Stojilkovic SS: Dependence of multidrug resistance protein-mediated cyclic nucleotide efflux on the background sodium conductance. Mol Pharmacol. 77: 270-279, 2010.
Zemkova H, Kucka M, Li S, Gonzalez-Iglesias AE, Tomic M, Stojilkovic SS: Characterization of purinergic P2X4 receptor channels expressed in anterior pituitary cells. Am J Physiol Endocrinol Metab. 298: E644-651, 2010.
Rokic MB, Tvrdonova V, Vavra V, Jindrichova M, Obsil T, Stojilkovic SS, Zemkova H: Roles of conserved ectodomain cysteines of the rat P2X4 purinoreceptor in agonist binding and channel gating. Physiol Res. 59: 927-935, 2010.
Stojilkovic SS, Kucka M, Li S, Gonzalez-Iglesias A, Tomic M, Zemkova H: Structural Insights into the Function of P2X4: an ATP-gated Cation Channel of Neuroendocrine Cells. Cell Mol Neurobiol 30: 1251-1258, 2010.

2009
Balík A, Jindrichová M, Bhattacharyya S, Zemková H: GnRH-I and GnRH-II-induced calcium signaling and hormone secretion in neonatal rat gonadotrophs. Phys Res 58 (5), 2009 (in press).
Jindrichova M, Vavra V, Obsil T, Stojilkovic SS, Zemkova H: Functional relevance of aromatic residues in the first transmembrane domain of P2X receptors. J Neurochem, 109: 923-934, 2009.

2008
Jelínková I, Vávra V, Jindrichová M, Obsil T, Zemková HW, Zemková H, Stojilkovic SS: Identification of P2X4 Receptor Transmembrane Residues Contributing to Channel Gating and Interaction with Ivermectin. Pflugers Arch, 456: 939-950, 2008.
Zemkova WH, Bjelobaba I, Tomic M, Zemkova H, Stojilkovic SS: Characterization of Depolarizing GABAA Receptor-Channels in Anterior Pituitary Cells. J Physiol 586: 3097–3111, 2008
Mazna P, Grycova L, Balik A, Zemkova H, Friedlova E, Obsilova V, Obsil T, Teisinger J:The role of proline residues in the structure and function of human MT2 melatonin receptor. J Pineal Res. 45:361-372. 2008 .
Zemková H, Balík A, Jindrichová M, Vávra V: Molecular Structure of Purinergic P2X Receptors and their Expression in the Hypothalamus and Pituitary. Physiol Res. 2008 May 13. Phys Res 57: S23-S38, 2008.
Zemkova H, Balik A, Mazna P: Melatonin Inhibition of Gonadotropin-Releasing Hormone-Induced Calcium Signaling and Hormone Secretion in Neonatal Pituitary Gonadotrophs. Chapter 5, pp.59-82. In: Haldar C, Singaravel M, Pandi-Perumal SR, Cardinali DP eds. Experimental Endocrinology and Reproductive Biology. Georgetown, TX, ISBN 978-1-57808-518-7; February 2008; 335 pages, © 2008 LANDES BIOSCIENCE

2007
Zemkova H, Yan Z, Liang Z, Jelinkova I, Tomic M., Stojilkovic SS (2007) Role of Aromatic and Charged Ectodomain Residues in the P2X4 Receptor Functions. J Neurochem 102:1139-1150, 2007.

2006
Zemkova H, Balik A, Jiang Y, Kretschmannova K, Stojilkovic SS (2006) Roles of purinergic P2X receptors as pacemaking channels and modulators of calcium-mobilizing pathway in pituitary gonadotrophs. Mol Endocrinol 20:1423-1436.
Gonzalez-Iglesias AE, Jiang Y, Tomic M, Kretschmannova K, Andric SA, Zemkova H, Stojilkovic SS (2006) Dependence of electrical activity and calcium influx-controlled prolactin release on adenylyl cyclase signaling pathway in pituitary lactotrophs. Mol Endocrinol 20:2231-2246.
Jelinkova I, Yan Z, Liang Z, Moonat S, Teisinger J, Stojilkovic SS, Zemkova H (2006) Identification of P2X(4) receptor-specific residues contributing to the ivermectin effects on channel deactivation. Biochem Biophys Res Commun 349:619-625.
Kretschmannova K, Gonzalez-Iglesias AE, Tomic M, Stojilkovic SS (2006) Dependence of hyperpolarisation-activated cyclic nucleotide-gated channel activity on basal cyclic adenosine monophosphate production in spontaneously firing GH3 cells. J Neuroendocrinol 18:484-493.
Koshimizu TA, Kretschmannova K, He ML, Ueno S, Tanoue A, Yanagihara N, Stojilkovic SS, Tsujimoto G (2006) Carboxyl-terminal splicing enhances physical interactions between the cytoplasmic tails of purinergic P2X receptors. Mol Pharmacol 69:1588-1598.
Gonzalez-Iglesias AE, Kretschmannova K, Tomic M, Stojilkovic SS (2006) ZD7288 inhibits exocytosis in an HCN-independent manner and downstream of voltage-gated calcium influx in pituitary lactotrophs. Biochem Biophys Res Commun 346:845-850.

2005
Stojilkovic SS, Tomic M, He ML, Yan Z, Koshimizu T, Zemkova H (2005) Molecular Dissection of Purinergic P2X Receptor-Channels. Ann N Y Acad Sci 1048, 116-130.
Kretschmannova K, Svobodova I, Balik A, Mazna P & Zemkova H (2005) Circadian Rhythmicity in AVP Secretion and GABAergic Synaptic Transmission in the Rat Suprachiasmatic Nucleus. Ann N Y Acad Sci 1048, 103-15.
Mazna P, Berka K, Jelinkova I, Balik A, Svoboda P, Obsilova V, Obsil T, Teisinger J (2005) Ligand binding to the human MT2 melatonin receptor: the role of residues in transmembrane domains 3, 6, and 7. Biochem Biophys Res Commun 332, 726-34.
Stojilkovic SS, Zemkova H & Van Goor F (2005) Biophysical basis of pituitary cell type-specific Ca2+ signaling-secretion coupling. Trends Endocrinol Metab 16, 152-9.
Zemková H, Balík A, Stojilkovic SS (2005) Expression and Signal Transduction Pathways of Melatonin Receptors in Pituitary. Chapter 8, In: Pandi-Perumal SR, Cardinali DP eds. Melatonin: Biological Basis of its Function in Health and Disease. Georgetowen, TX Landis Bioscience, 1-19, http://www.eurekah.com

2004
Balik A, Kretschmannova K, Mazna P, Svobodova I & Zemkova H (2004) Melatonin action in neonatal gonadotrophs. Physiol Res 53 Suppl 1, S153-66.
Kretschmannova K & Zemkova H (2004) Characterization of neuromuscular transmission in mice with progressive motoneuronopathy. Physiol Res 53, 541-8.
Mazna P, Obsilova V, Jelinkova I, Balik A, Berka K, Sovova Z, Ettrich R, Svoboda P, Obsil T & Teisinger J (2004) Molecular modeling of human MT2 melatonin receptor: the role of Val204, Leu272 and Tyr298 in ligand binding. J Neurochem 91, 836-42.
Wise PM, Radil T & Wysocki CJ (2004) Temporal integration in nasal lateralization and nasal detection of carbon dioxide. Chem Senses 29, 137-42.
Zemkova H, Balik A, Kretschmannova K, Mazna P & Stojilkovic SS (2004) Recovery of Ins(1,4,5)-trisphosphate-dependent calcium signaling in neonatal gonadotrophs. Cell Calcium 36, 89-97.
Zemkova H, He ML, Koshimizu TA & Stojilkovic SS (2004) Identification of ectodomain regions contributing to gating, deactivation, and resensitization of purinergic P2X receptors. J Neurosci 24, 6968-78.

2003
He ML, Zemkova H, Koshimizu TA, Tomic M & Stojilkovic SS (2003) Intracellular calcium measurements as a method in studies on activity of purinergic P2X receptor channels. Am J Physiol Cell Physiol 285, C467-79.
He ML, Zemkova H & Stojilkovic SS (2003) Dependence of purinergic P2X receptor activity on ectodomain structure. J Biol Chem 278, 10182-8.
Kretschmannova K, Svobodova I & Zemkova H (2003) Day-night variations in zinc sensitivity of GABAA receptor-channels in rat suprachiasmatic nucleus. Brain Res Mol Brain Res 120, 46-51.
Svobodova I, Vanecek J & Zemkova H (2003) The bidirectional phase-shifting effects of melatonin on the arginine vasopressin secretion rhythm in rat suprachiasmatic nuclei in vitro. Brain Res Mol Brain Res 116, 80-5.
Wise PM, Wysocki CJ & Radil T (2003) Time-intensity ratings of nasal irritation from carbon dioxide. Chem Senses 28, 751-60.
Wysocki CJ, Cowart BJ & Radil T (2003) Nasal trigeminal chemosensitivity across the adult life span. Percept Psychophys 65, 115-22 .