With the aim to facilitate high-throughput genome profiling and genetic mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five important species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum. The DArTFest array contains 7680 probes derived from methyl-filtered genomic representations.
The potential use of the DArTFest array:
The databasis for DArTFest array is freely available (http://bioinf.scri.ac.uk/germinate_grasses).
Kopecký, D., Bartoš, J., Lukaszewski, A.J., Baird, J.H., Černoch, V., Kölliker, R., Rognli, O.A., Blois, H., Caig, V., Lübberstedt, T., Studer, B., Shaw, B., Doležel, J. and Kilian, A. (2009). Development and mapping of DArT markers within the Festuca – Lolium complex. BMC Genomics 10:473.
Kopecký, D., Bartoš, J., Christelová, P.,Černoch, V., Kilian, A., Doležel, J. (2011). Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array. Theoretical and Applied Genetics 122:355–363.
Bartoš, J., Sandve, S.R., Kölliker, R., Kopecký, D., Stočes, Š., Christelová, P., Østrem, L., Larsen, A., Kilian, A., Rognli, O.A. and Doležel, J. (2011). Genetic mapping of DArT markers in the Festuca–Lolium complex and their use in freezing tolerance association analysis. Theoretical and Applied Genetics 122:1133–1147.
Baird, J., Kopecký, D., Lukaszewski, A.J., Green, R.L., Bartoš, J. and Doležel, J. (2012). Genetic Diversity of Turf-Type Tall Fescue Using Diversity Arrays Technology. Crop Science doi: 10.2135/cropsci2011.02.0106.
