The biophysical events responsible for electrical and mechanical activity of cardiac cells under physiological and pathophysiological conditions are investigated. The program includes the exploration of specific conditions underlying development of irregular excitations in cardiac cells (the cause of cardiac arrhythmias) and exploration of possibilities of pharmacological interventions with therapeutic effects. The principal method of this research is computational modelling using mathematical models of cardiac cells formulated on the basis of experimental data obtained from electrophysiological experiments in laboratories of our and foreign collaborating institutions.

Collaboration:

Structural scheme of the mathematical model of a rat cardiac cell.
From Pásek et al.: Eur. Biophys. J., 2012, Vol. 41, p. 491-503.
 

Related papers:
Pásek M., Šimurda J., Orchard C.H.: Effect of Ca²⁺ efflux pathway distribution and exogenous Ca²⁺ buffers on intracellular Ca²⁺ dynamics in the rat ventricular myocyte: a simulation study. Biomed. Res. Int., 2014, Vol. 2014, ID 920208.

Hrabcová D., Pásek M., Šimurda J., Christé G.: Effect of ion concentration changes in the limited extracellular spaces on sarcolemmal ion transport and Ca2+ turnover in a model of human ventricular cardiomyocyte. Int. J. Mol. Sci., 2013, Vol. 14, p. 24271-24292.

Pásek M., Šimurda, J., Orchard, C.H.: Role of t-tubules in the control of trans-sarcolemmal ion flux and intracellular Ca²⁺ in a model of the rat cardiac ventricular myocyte. Eur. Biophys. J., 2012, Vol. 41, p. 491-503. Matlab code of the model

Kilianová A., Bébarová M., Beránková K., Opatřilová R., Pásek M., Bartošová L.: Effect of newly synthesized compounds 44Bu and 444 on QRS -complex width and fast sodium current: differences between isomers. Acta Vet. Brno, 2010, Vol. 79, p. 41 -49.

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Orchard C.H., Pásek M., Brette F.: The role of mammalian cardiac t-tubules in excitation-contraction coupling: experimental and computational approaches (review). Exp. Physiol., 2009, Vol. 94, p. 509-519.

Bébarová M., Matejovič P., Pásek M., Jansová D., Šimurdová M., Nováková M., Šimurda J.: Effect of antipsychotic drug perphenazine on fast sodium current and transient outward potassium current in rat ventricular myocytes. Naunyn-Schmied. Arch. Pharmacol., 2009, Vol. 380, p.125-133.

Pásek M., Šimurda J., Christé G., Orchard C. H.: Modelling the cardiac transverse-axial tubular system (review). Prog. Biophys. Mol. Biol., 2008, Vol. 96, p. 226-243.

Pásek M., Brette F., Nelson A., Pearce C., Qaiser A., Christé G., Orchard C.H.: Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes. Prog. Biophys. Mol. Biol., 2008, Vol. 96, p. 244-257.

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Christé G., Chahine M., Chevalier P., Pásek M.: Changes in action potentials and intracellular ionic homeostasis in a ventricular cell model related to a persistent sodium current in SCN5A mutations underlying LQT3. Prog. Biophys. Mol. Biol., 2008, Vol. 96, p. 281-293.

Pásek M., Šimurda J., Christé G.: The functional role of cardiac T-tubules in a model of rat ventricular myocytes. Phil. Trans. R. Soc. A., 2006, Vol. 364, p. 1187-1206. Matlab code of the model

Bébarová M., Matejovič P., Pásek M., Nováková M.: Effect of haloperidol on transient outward potassium current in rat ventricular myocytes. Eur. J. Pharm., 2006, Vol. 550, p. 15-23.

Bébarová M., Matejovič P., Pásek M., Šimurdová M., Šimurda J.: Effect of ajmaline on transient outward current in rat ventricular myocytes. Gen. Physiol. Biophys., 2005, Vol. 24, p. 27-45.

Bébarová M., Matejovič P., Pásek M., Šimurdová M., Šimurda J.: Effect of ajmaline on action potential and ionic currents in rat ventricular myocytes. Gen. Physiol. Biophys., 2005, Vol. 24, p. 311-325.

Pásek M., Šimurda J.: Quantitative modelling of interaction of propafenone with sodium channels in cardiac cells. Med. Biol. Eng. Comput., 2004, Vol. 42, p. 151-157.

Pásek M., Christé G., Šimurda J.: A Quantitative model of the cardiac ventricular cell incorporating the transverse-axial tubular system. Gen. Physiol. Biophys., 2003, Vol. 22, p. 355-368.

Šimurda J., Šimurdová M., Pásek M., Bravený P.: Quantitative analysis of cardiac electrical restitution. Eur. Biophys. J., 2001, Vol. 30, p. 500-514.