Laboratory of Stress Physiology
Field of research
The research of the laboratory is focused on plant responses to different environmental stresses (especially those connecting with climate global change: elevated CO2 concentration, drought, and UV-radiation, and on heat or chilling stress, and heavy metal) and to biotic stresses (ageing, high content of endogenous cytokinins, viral infection) acting individually or in different combinations.
The changes in physiological and structural characteristics are determined on the level of whole plant and leaf, cellular, or subcellular level. We determine parameters of gas exchange, water regime, composition and function of photosynthetic apparatus and other cell organelles, development of reactive oxygen species, oxidative damage and induction of biosynthesis of protecting pigments and antioxidants. Furthermore, the changes in metabolism of reactive nitrogen species during natural and stress induced senescence are investigated.
Not only immediate responses to environmental factors are followed but also acclimations and de-acclimations to stresses and after their relief.
Important direction of our research is to determine the effect of phytohormones abscisic acid and cytokinins (endogenous content and/or application) on plant responses to stresses.
Our lab offers certain methods in frame of cooperation or on a contract:
- detection of oxidative damage (to lipids and/or proteins),
- antioxidative defence enzymes (catalase, superoxide dismutase, ascorbate peroxidase, glutathione reductase)
- low molecuar antioxidants (ascorbate, glutathione)
- phenolic compounds, anthocyanins
- pigments (chlorophylls, carotenoids)
Workers
head of the laboratorysenior scientist
- RNDr Naďa Wilhelmová CSc
scientist
- RNDr. Helena Synková CSc.
scientific assistant
- Mgr. Daniel Haisel Ph.D.
postdoc
- Mgr. Renáta Schnablová Ph.D.
maternity leavepostgraduate student
- Mgr. Petra Spoustová
technician
former employee
- RNDr Tomáš Gichner DrSc
- Ing. Dagmar Procházková PhD.
Research projects
- Nitrosative stress during natural and zinc induced senescence , GA ČR , Dagmar Procházková
- Fotoinhibice a oxidativní stres v průběhu aklimatizace in vitro pěstovaných rostlin , GA ČR , Jana Pospíšilová
- Změny v projevu heteróze u kukuřice v důsledku sucha: proč kříženci reagují odlišně než rodiče? , GA ČR , Naďa Wilhelmová
Publications
2003
- In Bioassays in Plant Cells for Improvement of Ecosystem and Human Health. Katowice 123-130 2003
- MUTATION RESEARCH - GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 535 187-193 2003 Fulltext: public PDF link
- MUTATION RESEARCH - GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 538 171-179 2003 Fulltext: public PDF link
- PHOTOSYNTHETICA 41 [1] 117-126 2003
- PHOTOSYNTHETICA 41 [3] 357-363 2003 Fulltext: public PDF link
2001
- BIOLOGIA PLANTARUM 44 321-324 2001
- PLANT SCIENCE 161 695-701 2001
1998
- Photosynthesis: Mechanisms and Effects . Pp2233-2236 1998
- PHYSIOLOGIA PLANTARUM 102 155-162 1998