Abstract:
The aim of the proposed research is to investigate the adsorption and transfer of biomedically important polyions at the polarizable interface between two immiscible electrolyte solutions, and to develop and apply the novel polyion sensing strategy. As apart the research programme, the interfacial behaviour of a series of polyaminoacids (e.g. poly-L-lysine) and substituted polysaccharides (e.g. heparin) dissociating into polyions in the solution will be studied by electrochemical and optical methods.Cyclic voltammetry and impedance spectroscopy will be used to measure the interfacial transfer kinetics and the double-layer charging as the potential-dependent phenomena. Computer simulations will be employed as a tool enabling to distinguish amongvarious mechanistic models. Adsorption data will be inferred with the help of the video-image pendant-drop and quasi-elastic laser light scattering methods.