The project is aimed at development and application of new approaches for monitoring of transport of selected xenobiotics and other biologically important compounds (e.g., drugs, hazardous elements, their ligands, agrochemically important compound as pesticides) across biomimetic membranes. It will be divided into two main parts: a) Development of reliable and sensitive analytical methods for detection and determination of above mentioned substances, which can be transported across phospholipid membranes, b) Development of methods for identification of transporting ways across which these substances are transported. The attention will be paid to the development and application of new sensors, based on nontraditional electrode materials (amalgams, nanostructured materials, etc.) with the aim to overcome limitations of traditional electrodes and to apply novel separation and preconcentration procedures. It is intended to employ electrochemical (voltammetric, EIS, etc.), chromatographic and mass spectrometric methods (GC-MS; HPLC-MS), immunochemical and microscopic methods.