The 80th Prague Meeting on Macromolecules, this time focused on Self-assembly in the world of polymers, took place in Prague on July 10–14, 2016. Its participants discussed theoretical aspects of polymer self-assembly, advanced methods of preparing and characterizing self-assembling macromolecular systems; special attention was paid to biomimetic self-assembled nanostructures as well as to biological and technical applications of self-assembled macromolecular systems.