Monoclonal antibodies available from the Laboratory of molecular immunology

 

The specificities of the mAbs marked as (W) were verified by international HLDA workshops.

 

 

Antigen

Antibody

Isotype

Note

CD2

MEM-65 (W)

IgG1

unique epitope

 

MEM-69

IgG1

 

CD3 (e)

MEM-57 (W)

IgG2a

can be used clinically (immunosuppression)

 

MEM-92 (W)

IgM

activating

CD4

MEM-16 (W)

IgM

epitope in D1-D2

 

MEM-115 (W)

IgG2a

epitope in D1; blocks gp120

 

MEM-241

IgG1

positive in W. blotting (non-red.)

 

MEM-242

IgG1

weakly positive in W. blotting (non-red.)

CD5

MEM-32 (W)

IgG1

positive in W. blotting (non-red.)

 

MEM-128 (W)

IgM

 

 

MEM-247

IgG1

positive in W. blotting (non-red.)

CD6

MEM-98

IgG1

positive in W. blotting (non-red.)

 

MEM-100

IgG1

 

CD7

MEM-186 (W)

IgG

positive in W. blotting (non-red.)

CD8

MEM-31 (W)

IgG2a

 

 

MEM-87 (+ several others)

IgG1

epitope similar to MEM-31

 

MEM-146 (W)

IgG1

epitope different from MEM-31

CD9

MEM-62 (W)

IgG1

 

 

MEM-192 (W)

IgM

positive in W. blotting (non-red.)

CD10 (CALLA)

MEM-78 (W)

IgG1

positive in W. blotting (nen-red.)

CD11a (LFA-1a)

MEM-25 (W)

IgG1

 

 

MEM-30 (W)

IgG1

 

 

MEM-83 (W)

IgG1

activates LFA-1 (to high affinity)

 

MEM-94

IgG1

blocks binding to ICAM-1

 

MEM-95 (W)

IgG1

blocks binding to ICAM-1

 

MEM-144

IgG1

 

 

MEM-152

IgG2b

 

 

MEM-177

IgG1

epitope similar to MEM-83

CD11b

MEM-170 (W)

IgG1

blocks binding to ICAM-1

 

MEM-174 (W)

IgG2a

 

CD14

MEM-15 (W)

IgG1

positive in W. blotting (non-red.)

 

MEM-18 (W)

IgG1

positive in W. blotting (non-red.) blocks binding of LPS-LBP to CD14

CD15

MEM-157

IgM

 

 

MEM-158

IgM

 

 

MEM-167

IgM

 

CD16

MEM-154 (W)

IgG1

positive in W. blotting (non-red.) recognizes a polymorphism in CD16A (NK-form)

 

MEM-168

IgM

 

CD17

MEM-74 (W)      (+ several others)

IgM

 

CD18 (LFA-1b)

MEM-48

IgG1

positive in W. blotting (non-red.) induces high-affinity state of LFA-1

 

MEM-148

IgG1

unique epitope accessible in free CD18 only; activating mAb

CD20

MEM-97 (W)

IgG1

 

CD22b

MEM-01

IgG1

positive in W. blotting (non-red.)

CD25

MEM-140 (W)

IgM

 

 

MEM-141

IgM

 

 

MEM-145 (W)

IgM

 

 

MEM-181 (W)

IgG1

 

CD29

MEM-101A (W)

IgG1

positive in W. blotting (non-red.)

CD30

MEM-268

IgG

positive in W. blotting (non-red.)

CD31

MEM-05

IgG1

 

CD41

MEM-06

IgG1

 

CD43

MEM-59 (W)

IgG1

sialic acid-dependent epitope  positive in W. blotting

 

MEM-256

IgG1

positive in W. blotting (non-red.);        (atypical-epitope absent in myeloid cells)

 

MEM-257

IgG1

positive in W. blotting (non-red.)

CD44

MEM-85 (W)

IgG2b

positive in W. blotting (non-red.)

 

MEM-263

IgG1

strongly positive in W. blotting

CD45

MEM-28 (W)      (+ several others)

IgG1

positive in W. blotting (red., non-red.)        positive in paraffin sections

CD45RA

MEM-56 (W)

IgG2b

positive in W. blotting

 

MEM-93 (W)

IgG1

as MEM-56

 

MEM-109          (+ several others)

IgG1

 

 

 

 

 

CD45RB

MEM-55 (W)

IgG1

carbohydrate-dependent epitope

 

MEM-143

IgG1

 

CD46

MEM-258

IgG2a

positive in W. blotting (non-red.)

CD47

MEM-122 (W)

IgM

 

 

MEM-120 (W)

IgM

low affinity

 

MEM-133 (W)

Ig2a

low affinity

CD48

MEM-102 (W)

IgG1

positive in W. blotting (non-red.)

 

MEM-124 (W)

IgM

 

 

MEM-200

IgG1

positive in W. blotting

 

MEM-201

IgG1

 

 

MEM-204

IgG3

 

 

MEM-211

IgG2b

positive in W. blotting

 

MEM-212

IgG2b

 

 

MEM-213

IgG1

 

CD50

MEM-04

IgG1

epitope in D1; blocks DC-SIGN binding

 

MEM-171 (W)

IgG1

epitope in D2

CD53

MEM-53 (W)

IgG1

positive in W. blotting (non-red.)

CD54 (ICAM-1)

MEM-111(W)

IgG2a

positive in W. blotting (non-red.)

 

MEM-112 (W)

IgG1

as MEM-111 but weaker

CD55 (DAF)

MEM-118 (W)

IgM

 

CD56

MEM-188 (W)

IgG2a

positive in W. blotting (non-red.)

CD58

MEM-63 (W)

IgG1

epitope in D1

CD59

MEM-43 (W)

IgG2a

positive in W. blotting (non-red.)

 

MEM-43/5 (W)    (+ several others)

IgG2b

different epitope; strong in W. blotting  non-red.)

 

MEM-125 (W)

IgM

 

 

MEM-129

IgM

 

CD63

MEM-259

IgG1

positive in W. blotting (non-red.)

CD71 (TfR)

MEM-75 (W)

IgG1

does not block transferrin binding

 

MEM-105

IgG2a

does not block transferrin binding

 

MEM-189 (W)

IgG1

positive in W. blotting (non-red.)                 does not block transferrin binding

 

MEM-191

IgG

does not block transferrin binding

CD80

MEM-233

IgG1

positive in W. blotting (non-red.)

 

MEM-234

IgG1

positive in W. blotting

 

MEM-235

IgG1

positive in W. blotting

CD97

MEM-180 (W)

IgG1

 

CD98

MEM-108 (W)

IgG1

 

 

MEM-156 (W)

IgM

 

CD99R

MEM-131 (W)

IgM

positive in W. blotting

CD105

MEM-223              (+ several others)

IgG

positive in W. blotting (non-red.)

CD108

MEM-121 (W)

IgM

positive in W. blotting

 

MEM-150 (W)

IgM

as MEM-121; higher affinity

CD147

MEM-M6/1             (+ several others)

IgG1

positive in W. blotting (non-red.)

CD173

MEM-195 (W)

IgM

 

 

MEM-196 (W)

IgM

 

 

MEM-197 (W)

IgM

 

 

MEM-198 (W)

IgM

 

CD177

MEM-166 (W)

IgG1

positive in W. blotting (non-red.)

CD222

MEM-238 (W)

IgG1

positive in W. blotting (non-red.)

 

MEM-239

IgG1

 

 

MEM-240 (W)

IgG1

positive in W. blotting (non-red.)

CD300

MEM-260

IgG

positive in W. blotting (non-red.)

 

 

 

 

b2 microglobulin

B2M-01

IgG2a

positive in W. blotting (non-red.)

 

B2M-02

IgG1

positive in W. blotting (non-red.)

HLA-DR

MEM-12             (+ several others)

IgG1

in W. blotting recognizes only the ab dimer  (non-boiled sample)

HLA-DR+DP (b-chain)

MEM-136

IgG1

positive in W. blotting (non-red.; red.)

 

MEM-138

IgG2a

positive in W. blotting (non-red.; red.)

HLA-DR (a-chain)

MEM-137

IgG1

positive in W. blotting (non-red.)

MHC class II, human, mouse

SLE-01, -03, -04, -06

chicken IgY

positive in W. blotting

 

 

 

 

HLA-class I

MEM-147

IgG1

reacts in W. blotting (non-red.) with some allelic forms; very similar to W6/32)

HLA-class I

MEM-81

IgG1

 

 

MEM-119

IgM

 

 

MEM-123

IgG3

 

 

MEM-130

IgM

 

 

MEM-149

IgG1

reacts with an undefined polymorphic      determinant (present in most subjects)

 

MEM-155

IgG1

 

HLA-E

MEM-E/2             (+ several others)

IgG

positive in W. blotting (does not react with native molecule)

 

MEM-E/6, E/7, E/8

IgG1

react with the native molecule

HLA-G

MEM-G/1              (+ several others)

IgG1

positive in W. blotting and paraffin sections  (does not react with native molecule)

 

MEM-G/9             (+ several others)

IgG1

reacts with native molecule

Ig k-chains

MEM-09

IgG1

positive in W. blotting (non-red.; red.)

 

MEM-40

IgG1

positive in W. blotting (non-red.; red.)

TCR-Vb5-related (HPB-ALL)

MEM-262

IgG2a

positive in W. blotting (non-red.; red.)

Insulin (human, pig, bovine)

IN-05

IgG1

 

 

IN-06

IgG2b

 

C-peptide of human proinsulin

C-PEP-01

IgG1

 

a-fetoprotein (human)

AFP-01, 02, 03     -11,-12,-13,-14

all IgG1

different epitopes

Glutamate carboxypeptidase II (GCPII)

GCP-01, 02, 04

all IgG1

positive in W. blotting (GCP-02)

Phosphotyrosine

P-TYR-01

IgG1

positive in W. blotting (non-red.; red.)

 

P-TYR-02

IgG2a

positive in W. blotting (non-red.; red.)

Lck (human)

LCK-01    (peptide 22-36)

IgG1

positive in W. blotting (non-red.; red.)

Lck (human, mouse)

LCK-04    (peptide 22-36)

IgG1

positive in W. blotting (non-red.; red.)

Fyn (human and mouse)

FYN-01  (fragment aa7-197)

IgG1

positive in W. blotting (non-red.; red.)

Csk (human, mouse)

CSK-04 (fragment aa330-450)

IgG1

positive in W. blotting (non-red.; red.)

SLP-76 (human)

SLP-76/1
SLP-76/2
SLP-76/3

IgG1
IgG1
IgG2b

Positive in W. blotting (weak)
Positive in W. blotting
Positive in W. blotting, also pig, mouse

ZAP-70

ZAP-70/2
ZAP-70/3

IgG1

Positive in W. blotting

SKAP-HOM (human and mouse)

SKAP-HOM-01, 02, 03

IgG

Positive in W. blotting, human, mouse

 

 

 

 

TRAIL

TRAIL-01           (+ several others)

IgG

positive in W. blotting; some mAbs blocking

TRIM

TRIM-01 through TRIM-10

IgG2a

some positive in W. blotting, some reactive also with mouse

SIT

SIT-01 through SIT-04

IgG

some positive in W. blotting, some reactive also with mouse

Daxx

DAXX-01 through DAXX-04

IgG

positive in W. blotting

PAG

MEM-253

IgG2a

weak in W. blotting; for IP

 

MEM-255

IgG2a

positive in W. blotting

 

PAG-C1

IgG2b

C-terminal peptide; human, mouse PAG

 

PAG-C6

IgG2b

C-terminal peptide; human, mouse PAG

NTAL

NAP-3

IgG2a

Anti-peptide; positive in W. blotting

 

NAP-7

IgG1

Positive in W. blotting (human, mouse)

 

NAP-8

IgG1

Positive in W. blotting (human, mouse)

LAT

LAT-01

IgG1

Positive in W. blotting; poor with phosphorylated LAT

LIME

LIME-10 (and several others)

IgG2a

Positive in W. blotting

EBP50 (NHERF)

EBP-10

IgG2b

Positive in W. blotting