Head : Prof. Mikael Kubista, PhD.

Vlasta Korenková, MSc., Ph.D.

Doc. RNDr. Ladislav Pecen, CSc.

Veronika Prokopová, MD.

Marianna Romžová, MSc., Ph.D.

Vendula Rusňáková, MSc.

Jana Ševčíková-Jonáková, MSc.

Monika Šídova, MSc.

David Švec, MSc.

Aleš Tichopád, MSc., PhD.

Zuzana Ušiaková, MD.

Research topics

The laboratory develops techniques for applications of real-time RT-PCR for studies of biological phenomena. Examples include early development of Xenopus laevis, tooth formation, and single cell expression profiling. Among recent accomplishments we determined the temporal expression profiles of 16 developmental genes in 16 stages of Xenopus laevis and, in collaboration with Professor S. Bustin´s laboratory in London, we developed qPCR tomography to determine intracellular expression profiles. Another objective of the department is to develop advanced multivariate statistical methods for analysis of spatiotemporal qPCR expression profiles, and recently we published the first multiway real-time PCR expression profiling study. The work is collaboration with MultiD Analyses (http://www.multid.se/). We also support the Prague TATAA Biocenter (http://www.tataa.com/) in real-time PCR courses.

Recent papers

 

Mikael Kubista.
Intracellular Gene Expression Profiles Revealed with Real-time PCR Tomography
Research Spotlight: Gene Expression, 2009.

 

Stephen A. Bustin, Vladimir Benes, Jeremy A. Garson, Jan Hellemans, Jim Huggett, Mikael Kubista, Reinhold Mueller, Tania Nolan, Michael W. Pfaffl, Gregory L. Shipley, Jo Vandesompele,5 and Carl T. Wittwer.
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments.
Clinical Chemistry 55,4, 2009.

 

Kubista M.
What future holds for real-time PCR.
European Pharmaceutical Reviews Industry Focus, 2009.

 

Ferjentsik Z, Sindelka R, Jonak J.
Expression patterns of Src-family tyrosine kinases during Xenopus laevis development.
The International Journal of Developmental Biology. 2009;53(1):163-8.

 

M. W. Pfaffl, J. Vandesompele and M. Kubista.
Data Analysis Software: Chapter 5 in Real-Time PCR: Current Technology and Applications.
Publisher: Caister Academic Press. Editor: Julie Logan, Kirstin Edwards and Nick Saunders ISBN: 978-1-904455-39-4, 2009.

 

Kubista M, Eliasson J, Lennerĺs M, Andersson S and Sjöback R.
What is needed to measure a difference with real-time PCR?
Eppendorf BioNews Application Notes 2008.

Kubista M. Emerging real-time PCR applications. Drug Discovery World Summer 2008.

Pinzani P, Lind K, Malentacchi F, Nesi G, Salvianti F, Villari D, Kubista M, Pazzagli M, Orlando C.
Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase-quantitative polymerase chain reaction and immuno-quantitative polymerase chain reaction.
Hum Pathol. 2008 Jul 10. [Epub ahead of print]

Bergkvist A, Forootan A, Zoric N, Strombom L, Sjoback R, Kubista M.
Choosing a normalization strategy for RT-PCR.
Genetic Engineering & Biotechnology News. 2008 Jul; 28 (13):26

Niazi A, Yazdanipour A, Ghasemi J, Amini A, Bozorgzad S, Kubista M.
Spectrophotometric investigation of the acidity constants of fluorescein in various water-organic solvent media.
Chemical Engineering Communications. 2008; 195(10):1257-1268.

Li LZ, Lundkvist A, Andersson D, Wilhelmsson U, Nagai N, Pardo AC, Nodin C, Stahlberg A, Aprico K, Larsson K, Yabe T, Moons L, Fotheringham A, Davies I, Carmeliet P, Schwartz JP, Pekna M, Kubista M, Blomstrand, Maragakis N, Nilsson M, Pekny M.
Protective role of reactive astrocytes in brain ischemia.
Journal of Cerebral Blood Flow and Metabolism. 2008 Mar; 28(3):468-481.

Ståhlberg A, Elbing K, Andrade-Garda JM, Sjögreen B, Forootan A, Kubista M.
Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli.
BMC Genomics. 2008 Apr 16;9:17

Sindelka R, Jonák J, Hands R, Bustin SA, Kubista M.
Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte.
Nucleic Acids Res. 2008 Feb;36(2):387-92

Ciencialová A, Neubauerová T, Sanda M, Sindelka R, Cvačka J, Voburka Z, Buděšínský M, Kašička V, Sázelová P, Solínová V, Macková M, Koutek B, Jiráček J.
Mapping the peptide and protein immune response in the larvae of the fleshfly Sarcophaga bullata.
J Pept Sci. (2007)

I. M. Mackay, S. A. Bustin, J. M. Andrade, M. Kubista and T. P Sloots.
Quantification of Micro-Organisms: Not Human, Not Simple, Not Quick. Chapter 5 in Real-Time PCR in Microbiology: From Diagnosis to Characterization.
Publisher: Caister Academic Press. Editor: Ian M. Mackay Sir Albert Sakzewski Virus Research Centre, Queensland, Australia. ISBN: 978-1-904455-18-9 (2007).

M.G.H.M. Beld, C. Birch, P.A. Cane, W. Carman, E.C.J. Claas, J.P. Clewley, E. Domingo, J. Druce, C. Escarmis, R.A.M. Fouchier, V. Foulongne, M.G. Ison, L.C. Jennings, B. Kaltenboeck, I.D. Kay, M. Kubista, O. Landt, I.M. Mackay, J. Mackay, H.G.M. Niesters, M.D. Nissen, S. Palladino, N.G. Papadopoulos, A. Petrich, M,W. Pfaffl, W. Rawlinson, U. Reischl, N.A. Saunders, C. Savolainen-Kopra, O. Schildgen, G.M. Scott, M. Segondy, R. Seibl, T.P. Sloots, Y-W. Tang, R. Tellier and P.C.Y. Woo. Experts Roundtable: Real-Time PCR and Microbiology.
Chapter 10 in Real-Time PCR in Microbiology: From Diagnosis to Characterization. Publisher: Caister Academic Press. Editor: Ian M. Mackay Sir Albert Sakzewski Virus Research Centre, Queensland, Australia.
ISBN: 978-1-904455-18-9 (2007).

Andrade JM, Kubista M, Carlosena A, Prada D.
3-Way characterization of soils by Procrustes rotation, matrix-augmented principal components analysis and parallel factor analysis.
Anal Chim Acta. (2007), 603(1): 20-9.

M. Kubista, R. Sindelka, A. Tichopad, A. Bergkvist, D. Lindh, A. Forootan.
The Prime Technique. Real-time PCR data analysis.
G.I.T. Laboratory Journal (2007), 9-10, 33-35

L. Li, A. Lundkvist, D. Andersson, U. Wilhelmsson, N. Nagai, A. C. Pardo, C. Nodin, A. Sta°hlberg, K. Aprico, K. Larsson, T. Yabe, L. Moons, A. Fotheringham, I. Davies, P. Carmeliet, J. P. Schwartz, M. Pekna, M. Kubista, F. Blomstrand, N. Maragakis, M. Nilsson and M.Pekny.
Protective role of reactive astrocytes in brain ischemia.
Journal of Cerebral Blood Flow & Metabolism (2007), 1–14

Real-Time PCR in Microbiology: From Diagnosis to Characterization, Experts Roundtable: Real-Time PCR and Microbiology 

AAA Real-Time PCR Tech Guide - Volume 3

AAA Real-Time PCR Tech Guide - Volume 2

AAA Real-Time PCR Tech Guide - Volume 1

M. Kubista, B. Sjogreen, A. Forootan, R. Sindelka, J. Jonak, JM. Andrade.
Real-time PCR gene expression profiling.
European Pharmaceutical Reviews (2007), Vol. 1.