Synthesis of Peptides

We are interested in synthesis of peptides on solid phase. The principle of solid phase peptide synthesis as conceived by R.B.Merrifield is based on the idea that if the synthesized peptide is bound to an insoluble support then any unreacted reagents left at the end of any synthetic step can be removed by a simple wash procedure. Besides, such arrangement is amenable to automation. The peptides are synthesized by Fmoc chemistry. The reason is that the use of the base labile fluorenylmethyloxycarbonyl group (Fmoc) for -amino group protection enables relatively mild deprotection and cleavage from the resin. The peptide sequence was assembled by a solid-phase synthesizer ABI 433A (Applied Biosystems).