Research topics
Signalling mechanism of G-protein-coupled receptors for major neurotransmitters Structure-function relationship of metabotropic glutamate and GABAb receptors
Current Grant Support
GA AS CR IAA400400621 (2006-2010)
Ministry of Education, Youth and Sports o the Czech Republic LC06063 "FLUMBIOL" (2006-2010)
GA AS CR IAA500390701 (2007-2011)
GACR GA 303/08/1591 (2008-2010)
Most important recent papers
(2002-present; names of the lab members underlined)Havlickova, M., Prézeau, L., Duthey, B., Bettler, B., Pin, J.-P., Blahos, J.: The intracellular loops of the GB2 subunit are crucial for G-protein coupling of the heteromeric GABAB receptor. Mol. Pharmacol. 62: 343-350, 2002.
Havlickova, M., Blahos, J., Brabet, I., Liu, J., Hruskova, B., Prézeau, L., Pin, J.-P.: The second intracellular loop of metabotropic glutamate receptors recognizes C-termini of G protein beta-subunits J. Biol. Chem. 278: 35063-35070, 2003.
Iacovelli, L., Capobianco, L., Iula, M., Di Giorgi Gerevini, V., Picascia, A., Blahos, J., Melchiorri, D., Nicoletti, F., De Blasi, A.: Regulation of mGlu4 metabotropic glutamate receptor signaling by type-2 G-protein coupled receptor kinase (GRK2). Mol. Pharmacol. 65: 1103-10, 2004.
Goudet, C., Kniazeff, J., Hlavackova, V., Malhaire, F., Maurel, D., Acher, F., Blahos, J., Prezeau, L., Pin, J.-P.: Asymmetric functioning of dimeric metabotropic glutamate receptors disclosed by positive allosteric modulators J. Biol. Chem. 280: 24380-5, 2005.
Sinagra, M., Verrier, D., Frankova, D., Korwek, K. M. Blahos, J., Weeber, E. J., Manzoni, O. J., Chavis, P.: Reelin, very-low-density lipoprotein receptor, and apolipoprotein E receptor 2 control somatic NMDA receptor composition during hippocampal maturation in vitro. J. Neurosci. 25: 6127-36, 2005.
Brock, C., Boudier, L., Maurel, D., Blahos, J., Pin. J.-P.: Assembly-dependent surface targeting of the heterodimeric GABAB receptor is controlled by COPI, but not 14-3-3. Mol. Biol. Cell 16: 5572-5578, 2005.
Hlavackova, V., Kniazeff, J., Goudet, C., Zikova, A., Maurel, D., Vol, C., Trojanova, J., Prézeau, L., Pin, J.-P., Blahos, J.: Evidence for a single heptahelical domain being turned on upon activation of a dimeric GPCR. EMBO J. 24: 499-509, 2005.
Bertaso, F., Lill, Y., Airas, J. M., Espeut, J., Blahos, J., Bockaert, J., Fagni, L., Betz, H., El-Far, O.: MacMARCKS interacts with the metabotropic glutamate receptor type 7 and modulates G protein-mediated constitutive inhibition of calcium channels. J. Neurochem. 99: 288-98, 2006.
Kumpost J, Syrova Z, Kulihova L, Frankova D, Bologna JC, Hlavackova V, Prezeau L, Kralikova M, Hruskova B, Pin JP, Blahos J. Surface expression of metabotropic glutamate receptor variants mGluR1a and mGluR1b in transfected HEK293 cells. Neuropharmacology 55(4): 409-18, 2008.
Reviews
Pin, J-P., Kniazeff, J., Binet, V., Liu, J., Maurel, D., Galvez, T., Duthey, B., Havlickova, M., Blahos, J., Prézeau, L., Rondard, P.: Activation mechanism of the heterodimeric GABAB receptor. Biochem. Pharmacol. 68: 1565-1572, 2004.