Research topics
DNA damage response; DNA repair mechanisms; hereditary DNA repair disorders.
Current Grant Support
Grant Agency of the Czech Republic (2009-2013; grant No. GA204/09/0565): Role of RECQ5 DNA helicase in maintenance of genomic stability.
Most important recent papers
(2004-present; names of the lab members underlined)Garcia PL, Liu Y, Jiricny J, West SC, Janscak P. Human RECQ5beta, a protein with DNA helicase and strand-annealing activities in a single polypeptide. EMBO J 23: 2882-91, 2004. (PubMed)
Garcia PL, Bradley G, Hayes CJ, Krintel S, Soultanas P, Janscak P. RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases. Nucleic Acids Res 32: 3771-8, 2004. (PubMed)
Ramadan K, Shevelev IV, Maga G, Hübscher U. De novo DNA synthesis by human DNA polymerase lambda, DNA polymerase mu and terminal deoxyribonucleotidyl transferase. J Mol Biol 339: 395-404, 2004. (PubMed)
Toueille M, El-Andaloussi N, Frouin I, Freire R, Funk D, Shevelev I, Friedrich-Heineken E, Villani G, Hottiger MO, Hübscher U. The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair. Nucleic Acids Res 32: 3316-24, 2004. (PubMed)
Frouin I, Toueille M, Ferrari E, Shevelev I, Hübscher U. Phosphorylation of human DNA polymerase lambda by the cyclin-dependent kinase Cdk2/cyclin A complex is modulated by its association with proliferating cell nuclear antigen. Nucleic Acids Res 33: 5354-61, 2005. (PubMed)
El-Shemerly M, Janscak P, Hess D, Jiricny J, Ferrari S. Degradation of human exonuclease 1b upon DNA synthesis inhibition. Cancer Res 65: 3604-9, 2005. (PubMed)
Wu L, Chan KL, Ralf C, Bernstein DA, Garcia PL, Bohr VA, Vindigni A, Janscak P, Keck JL, Hickson ID. The HRDC domain of BLM is required for the dissolution of double Holliday junctions. EMBO J 24: 2679-87, 2005. (PubMed)
Cheok CF, Wu L, Garcia PL, Janscak P, Hickson ID. The Bloom's syndrome helicase promotes the annealing of complementary single-stranded DNA. Nucleic Acids Res 33: 3932-41, 2005. (PubMed)
Kanagaraj R, Saydam N, Garcia PL, Zheng L, Janscak P. Human RECQ5beta helicase promotes strand exchange on synthetic DNA structures resembling a stalled replication fork. Nucleic Acids Res 34: 5217-31, 2006. (PubMed)
Blasius M, Shevelev I, Jolivet E, Sommer S, Hübscher U. DNA polymerase X from Deinococcus radiodurans possesses a structure-modulated 3'-->5' exonuclease activity involved in radioresistance. Mol Microbiol 60: 165-76, 2006. (PubMed)
Hu Y, Raynard S, Sehorn MG, Lu X, Bussen W, Zheng L, Stark JM, Barnes EL, Chi P, Janscak P, Jasin M, Vogel H, Sung P, Luo G. RECQL5/Recql5 helicase regulates homologous recombination and suppresses tumor formation via disruption of Rad51 presynaptic filaments. Genes Dev 21: 3073-84, 2007. (PubMed)
Saydam N, Kanagaraj R, Dietschy T, Garcia PL, Pe.a-Diaz J, Shevelev I, Stagljar I, Janscak P. Physical and functional interactions between Werner syndrome helicase and mismatch-repair initiation factors. Nucleic Acids Res 35: 5706-16, 2007. (PubMed)
Jiao R, Harrigan JA, Shevelev I, Dietschy T, Selak N, Indig FE, Piotrowski J, Janscak P, Bohr VA, Stagljar I. The Werner syndrome protein is required for recruitment of chromatin assembly factor 1 following DNA damage. Oncogene 26: 3811-22, 2007. (PubMed)
Selak N, Bachrati CZ, Shevelev I, Dietschy T, van Loon B, Jacob A, Hübscher U, Hoheisel JD, Hickson ID, Stagljar I. The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase {delta}. Nucleic Acids Res 36: 5166-5179, 2008. (PubMed)
Ren H, Dou SX, Zhang XD, Wang PY, Kanagaraj R, Liu JL, Janscak P, Hu JS, Xi XG. The zinc-binding motif of human RECQ5beta suppresses the intrinsic strand-annealing activity of its DExH helicase domain and is essential for the helicase activity of the enzyme. Biochem J 412: 425-33, 2008. (PubMed)
Spycher C, Miller ES, Townsend K, Pavic L, Morrice NA, Janscak P, Stewart GS, Stucki M. Constitutive phosphorylation of MDC1 physically links the MRE11-RAD50-NBS1 complex to damaged chromatin. J Cell Biol 181: 227-40, 2008. (PubMed)
Lapkouski M., Panjikar S., Janscak P, Kuta-Smatanova I., Carey J., Ettrich R., Csefalvay E. Structure of the motor subunit and translocation model for EcoR124I restriction-modification complex. Nature Struct Mol Biol 16: 94-95, 2009. (PubMed)
Selak N, Bachrati CZ, Shevelev I, Dietschy T, van Loon B, Jacob A, Hübscher U, Hoheisel JD, Hickson ID, Stagljar I. The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta. Nucleic Acids Res 36: 5166-79, 2008. (PubMed)
Yodh JG, Stevens BC, Kanagaraj R, Janscak P, Ha T. BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation. EMBO J, 2009 Jan 22. [Epub ahead of print] (PubMed)
Reviews
Shevelev IV, Hübscher U. The 3'-->5' exonucleases. Nat Rev Mol Cell Biol 3:364-76, 2002. (PubMed)
Ramadan K, Shevelev I, Hübscher U. The DNA-polymerase-X family: controllers of DNA quality? Nat Rev Mol Cell Biol 5:1038-43, 2004. (PubMed)
Dietschy T, Shevelev I, Stagljar I. The molecular role of the Rothmund-Thomson-, RAPADILINO- and Baller-Gerold-gene product, RECQL4: recent progress. Cell Mol Life Sci 64: 796-802, 2007. (PubMed)