Cvikrová M., Malá J., Hrubcová M., Eder J., Foretová S.
JOURNAL OF PLANT DISEASES AND PROTECTION
115:
57-62,
2008
Keywords:
astringin, isorhapontin, Picea abies, piceid,
Abstract:
The aim of this work was to identify whether the Ascocalyx
abietina culture filtrate has the ability to induce changes in the
contents of phenolic substances that might be indicative for
the disease response of Norway spruce (Picea abies). We
focused on the accumulation of soluble and cell wall-bound
phenolics, stilbenes as well as extracellular peroxidase activities
elicited in the embryogenic cell cultures of Norway spruce
by A. abietina culture filtrate. Treatment of spruce cells with
fungal culture filtrate (5 and 20% v/v concentrations) evoked
an increase in the total contents of phenolic acids (represented
by the sum of free, methanol soluble ester- and glycoside-
bound phenolics and methanol insoluble cell-wall bound
phenolic esters). The challenge with filtrate was in particular
manifested in the increase (compared with the control) in the
contents of cell wall-bound phenolic acids (by about 100 and
130% in 5 and 20% filtrate, respectively) and soluble phenolic
glycosides (by 37 and 65% in 5 and 20% filtrate, respectively)
already 6 h after filtrate addition. Significantly decreased concentrations
of stilbene glycosides, isorhapontin, astringin and
piceid, were determined in filtrate-treated spruce cell cultures
6 and12 h after filtrate addition. The culture filtrate did not
influence significantly the extracellular peroxidase activity
12 h after filtrate addition. Reduced extracellular peroxidase
activity at 24 and 48 h in treated cells and decline in the
amounts of soluble phenolics coincided with the rate of
browning of filtrate-treated cells.
Fulltext: contact IEB authors
IEB authors: Milena Cvikrová,
Marie Hrubcová
Čeština
English