Research topics
Epithelial and epidermal compartments, liver, inflammation, metalloproteinases, kallikreins and their inhibitors, adhesion molecules, extracellular matrix proteins, cell-matrix interactions.
Current Grant Support
GAAV CR IAA500520812, Investigating the pathologic role of metalloproteinases in liver.
GACR 301/08/J053, Mouse models for investigation of biological functions of novel Kazal-type protease inhibitors.
Most important recent papers
(2002-present; names of the lab members underlined)Mauch S, Kolb C, Kolb B, Sadowski T, Sedlacek R. Matrix metalloproteinase-19 is expressed in myeloid cells in an adhesion-dependent manner and associates with the cell surface. J Immunol 168:1244-51, 2002. (PubMed)
Sadowski T, Dietrich S, Koschinsky F, Sedlacek R. Matrix metalloproteinase 19 regulates insulin-like growth factor-mediated proliferation, migration, and adhesion in human keratinocytes through proteolysis of insulin-like growth factor binding protein-3. Mol Biol Cell 14:4569-80, 2003. (PubMed)
Sadowski T, Dietrich S, Müller M, Havlickova B, Schunck M, Proksch E, Müller MS, Sedlacek R. Matrix metalloproteinase-19 expression in normal and diseased skin: dysregulation by epidermal proliferation. J Invest Dermatol 121:989-96, 2003. (PubMed)
Jirouskova M, Chereshnev I, Väänänen H, Degen JL, Coller BS. Antibody blockade or mutation of the fibrinogen gamma-chain C-terminus is more effective in inhibiting murine arterial thrombus formation than complete absence of fibrinogen. Blood 103:1995-2002, 2004. (PubMed)
Abel S, Hundhausen C, Mentlein R, Schulte A, Berkhout TA, Broadway N, Hartmann D, Sedlacek R, Dietrich S, Muetze B, Schuster B, Kallen KJ, Saftig P, Rose-John S, Ludwig A. The transmembrane CXC-chemokine ligand 16 is induced by IFN-gamma and TNF-alpha and shed by the activity of the disintegrin-like metalloproteinase ADAM10. J Immunol 172:6362-72, 2004. (PubMed)
Flick MJ, Du X, Witte DP, Jirouskova M, Soloviev DA, Busuttil SJ, Plow EF, Degen JL. Leukocyte engagement of fibrin(ogen) via the integrin receptor alphaMbeta2/Mac-1 is critical for host inflammatory response in vivo. J Clin Invest 113(11):1596-606, 2004. (PubMed)
Titz B, Dietrich S, Sadowski T, Beck C, Petersen A, Sedlacek R. Activity of MMP-19 inhibits capillary-like formation due to processing of nidogen-1. Cell Mol Life Sci 61:1826-33, 2004. (PubMed)
Palumbo JS, Talmage KE, Massari JV, La Jeunesse CM, Flick MJ, Kombrinck KW, Jirouskova M, Degen JL. Platelets and fibrin(ogen) increase metastatic potential by impeding natural killer cell-mediated elimination of tumor cells. Blood 105:178-85, 2005. (PubMed)
Sadowski T, Dietrich S, Koschinsky F, Ludwig A, Proksch E, Titz B, Sedlacek R. Matrix metalloproteinase 19 processes the laminin 5 gamma 2 chain and induces epithelial cell migration. Cell Mol Life Sci 62:870-80, 2005. (PubMed)
Beck IM, Müller M, Mentlein R, Sadowski T, Mueller MS, Paus R, Sedlacek R. Matrix metalloproteinase-19 expression in keratinocytes is repressed by transcription factors Tst-1 and Skn-1a: implications for keratinocyte differentiation. J Invest Dermatol 127:1107-14, 2007. (PubMed)
Jirouskova M, Jaiswal JK, Coller BS. Ligand density dramatically affects integrin alpha IIb beta 3-mediated platelet signaling and spreading. Blood 109:5260-9, 2007. (PubMed)
Beck IM, Rückert R, Brandt K, Mueller MS, Sadowski T, Brauer R, Schirmacher P, Mentlein R, Sedlacek R. MMP19 is essential for T cell development and T cell-mediated cutaneous immune responses. PLoS ONE 3:e2343, 2008. (PubMed)
Reviews
Emeis JJ, Jirouskova M, Muchitsch EM, Shet AS, Smyth SS, Johnson GJ. 2007. A guide to murine coagulation factor structure, function, assays, and genetic alterations. J Thromb Haemost 5:670-9. (PubMed)
Jirouskova M, Shet AS, Johnson GJ. 2007. A guide to murine platelet structure, function, assays, and genetic alterations. J Thromb Haemost 5:661-9. (PubMed)
Other results
Department is offering embryo manipulating services including DNA pronuclear microinjections, sperm freezing and archiving, in vitro fertilization (IVF), embryo isolations, rederivation of mouse lines/strains, vasectomy, etc.