Laboratory of Tissue Engineering

A liposome-based controlled drug delivery and nutrient supply system to deliver bioactive substances directly into defects was developed. The application of the novel technology of coaxial spinning for the production of smart nanofibers is intensively studied, especially in combination with liposomes, with the aim of developing suitable systems for controlled drug delivery. This advanced drug delivery system is mediated with liposome- and immunoliposomeenriched nanofibers and controlled by ultrasound sonication and shock-waves.

Artificial tissue implantation is another research topic. A novel approach was found to improve chondrocyte proliferation, nutrition and redifferentiation capacity, at the same time providing appropriate mechanical stability. The constructed scaffolds seeded with autologous chondrocytes can successfully heal osteochondral defects in experimental animals (rabbits and miniature pigs). Pre-clinical studies, following Good Laboratory Practice, are currently in progress.

The modern approach of computer modeling is applied for predicting the structural properties of cells and tissues, including protein dynamics. Computer modeling, based on homology and similarity with proteins of known structure, is focused on protein structure determination and molecular dynamics simulation. Advanced studies of the molecular mechanism of Na⁺/K⁺-ATPase phosphorylation and of the structure and dynamics of the ATP-binding site on Na⁺/K⁺-ATPase are carried out. The relation between Na⁺/K⁺-ATPase structure, function and diseases (specifically familial hemiplegic migraine – FHM2) is also investigated.

High on our priority list is also the accelerated transfer of newly developed technologies and know-how into clinical practice.