Laboratory of Tissue Engineering
Research topics
The research is concentrated on the development of novel three-dimensional scaffolds utilizing biodegradable materials. Textiles, both woven and non-woven, as well as composite scaffolds are generated mainly employing a nanofiber-based approach and applied separately or in combination with an isotropic gel. Grafts based on autologous chondrocytes and mesenchymal stem cells are used for tissue defect regeneration (namely cartilage and bone). A special technique for the rapid evaluation of biomechanical properties in miniature tissue pieces was developed.
Scaffolds and cell seeding.
Electron microscopy of a cross-linked gelatin scaffold and confocal microscopy of a chondrocyte-seeded scaffold.
Histology of an osteochondral defect 6 weeks after implantation demonstrates the capability of a composite hyaluronan/type I collagen/fibrin scaffold to regenerate rabbit-knee cartilage.
Hematoxylin–eosin staining.
Glycosaminoglycan detection by Alcian blue staining and PAS reaction. Immunohistochemical detection of type II collagen.
