Dissecting the complex wheat genome to smaller parts will simplify its sequencing, analysis, and positional gene cloning. We propose to construct 1D, 4D, and 6D-specific BAC DNA library suitable for construction of physical maps. The results and materials that will be obtianed will facilitate the analysis of evolution of the hexaploid wheat genome. The new genomic resources will facilitate cloning of wheat genes underlining resistance to biotic and abiotic stresses, as well as yield and quality. The BAC libraries and physical maps will provide a basis for wheat genome sequencing.
