Šimková, Hana; Janda, Jaroslav; Hřibová, Eva; Šafář, Jan; Doležel, Jaroslav
PLANT, SOIL AND ENVIRONMENT
53 [10]:
437-441,
2007
Keywords:
Cot; BAC library; wheat
Abstract:
Sequencing of cereal genomes is not a feasible task due to their large size and high content of repetitive DNA sequences. There are two basic approaches to simplify analysis of such genomes: reduced representation approaches, such as EST sequencing, methyl filtration and Cot-based cloning and sequencing; on the other side there is analysis of genomes in a step-wise manner, e.g. through creation of chromosome-specific genomic resources. Combination of both approaches – i.e. Cot-based cloning and sequencing of DNA obtained from a chromosome-arm-specific BAC library – was tested in this work.
IEB authors: Jaroslav Doležel,
Eva Hřibová,
Jan Šafář,
Hana Šimková
Čeština
English