Supervisor ((Consultant)) | Jan Dohnálek Consultant Jarmila Dušková |
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Topic | 3D structure analysis of chitinolytic enzymes of bacterial microflora of the gastro-intestinal tract. |
Annotation | Bacteria inhabiting human gastro-intestinal tract express several enzymes capable of chitin cleavage. This complex of chitinolytic enzymes degrades chitin-containing cell walls of fungi and contributes to human health. A detailed study of enzymes of one of these bacteria provides important characteristics and properties of the molecules, including determination of their three-dimensional structure by the means of x-ray diffraction. The work is focused on enzyme isolation from bacterial extracts, their purification, activity assays,crystallization and determination of their three-dimensional structure by x-ray diffraction analysis followed by computational analysis of the obtained results. |
Institute of Macromolecular Chemistry AS CR, v.v.i.
Heyrovského nám. 2
CZ-162 06 Praha 6
Czech Republic
phone:+420 296 809 111
fax:+420 296 809 410