Schnablová R., Synková H., Vičánková A., Burketová L., Eder J., Cvikrová M
PLANT PHYSIOLOGY AND BIOCHEMISTRY
44:
526-534,
2006
Keywords:
Pssu-ipt tobacco; Phenolic acids; Cytokinins; In vitro cultivation; Peroxidases
Abstract:
We present evidence that overproduction of endogenous cytokinins (CK) caused stress response in non-rooting Pssu-ipt transgenic tobacco
(Nicotiana tabacum L.) grown in vitro. It was demonstrated by overaccumulation of phenolic compounds, synthesis of pathogenesis related
proteins (PR proteins), and increase in peroxidase (POD) activities. Immunolocalization of zeatin and also PR-1b protein on leaf cryo-sections
proved their accumulation in all mesophyll cells of transgenic tobacco contrary to control non-transgenic plants. Intensive blue autofluorescence
of phenolic compounds induced by UV in cross-sections of leaf midrib showed enhanced contents of phenolics in transgenic tobacco compared
with controls, nevertheless, no significant difference between both plant types was found in leaf total lignin content. Transgenic plantlets exhibited
higher peroxidase activities of both soluble and ionically bound fractions compared with controls. HPLC analysis of phenolic acids confirmed
the increase in all phenolic acids in transgenic tobacco except for salicylic acid (SA). The effect of high phenolic content on rooting of
transgenic tobacco is discussed.
IEB authors: Lenka Burketová,
Milena Cvikrová,
Helena Synková
Čeština
English