Šafář, J., Šimková, H., Kubaláková, M., Číhalíková, J., Suchánková, P., Bartoš, J., Doležel, J.
CYTOGENETIC AND GENOME RESEARCH
129:
211-223,
2010
Klíčová slova:
BAC library, Chromosome, DNA markers, Flow sorting, Physical mapping, Wheat
Abstrakt:
The large bread wheat genome (1C 17 Gbp) contains a preponderance
of repetitive DNA and the species is polyploid.
These characteristics together serve to hamper the molecular
analysis of the wheat genome. Its complexity can, however,
be reduced by using flow cytometry to isolate individual
chromosomes, and these can be exploited to construct
chromosome-specific BAC libraries. Such libraries simplify
the task of physical map construction, positional cloning and
the targeted development of genetic markers. Rapid improvements
in the efficiency and cost of DNA sequencing
provide an opportunity to contemplate sequencing the
wheat genome by preparing sequence-ready physical maps
for each chromosome or chromosome arm in turn. The quality
of the chromosome-specific libraries depends on their
chromosome coverage and the mean insert size. First-generation
libraries suffered from a relatively low mean insert
size, but improvements to the protocol have generated a
second wave of libraries with a significantly increased mean
insert size and better chromosome coverage. Each chromosome (arm)-specific library is composed of a manageable
number of clones, and so represents a practical tool in the
area of wheat genomics.
Fulltext: kontaktujte autory z ÚEB
Autoři z ÚEB: Jan Bartoš,
Jarmila Číhalíková,
Jaroslav Doležel,
Marie Kubaláková,
Jan Šafář,
Hana Šimková,
Pavla Suchánková
Čeština
English