Projects
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Metalloenzyme inhibitorsThis project involves the design, synthesis and biological characterization of transition-state mimicking inhibitors of metalloenzymes. At presence, we are synthesizing inhibitors for betaine-homocysteine S-methyltransferase (BHMT) and for methionine aminopeptidases of type I and II (MetAP I and II). BHMT is a mammalian enzyme involved in the synthesis of methionine from betaine and homocysteine and has an important role in the metabolism of sulfur amino acids in liver and kidney. MetAPs are ubiquitous enzymes found in both eukaryotic and prokaryotic cells. These enzymes are involved in the selective removal of the initiator N-terminal methionine residue from newly synthesized polypeptide chains. |
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Insulin analogsWe prepare new insulin analogs with modifications in the C-terminus of the B-chain, which is important binding site for the insulin receptor. Better understanding of the interaction of insulin with its receptor may help in the treatment of diabetes and many other diseases related to insulin pathways. |
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ProteomicsWe use 2D-electrophoresis and different separation methods for the identification of protein markers in various breast cancer cell lines and for the identification of new antimicrobial compounds from the larvae of fleshly Neobellieria bullata. |
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Peptides involved in food intake regulationOur research is focused on peptides such as CART (cocaine and amphetamine regulated transcript) peptides involved in the regulation of food intake and processes related to obesity. Our project aims at elucidating the regulatory pathways and relationship of peptides involved in feeding related processes, both in vivo and in vitro. |