Plchova H., Moravec T., Dedic P., Cerovska N.
JOURNAL OF PHYTOPATHOLOGY
doi: 10.1111/j.1439-0434.2010.01740.x:
,
2010
Keywords:
Potato leafroll virus, recombinant viral antigen, antibody production
Abstract:
Vector pMPM-A4Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used
for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli.
Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)–fragments RdRp43-616 and RdRp304-537
were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system
using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged
PLRV proteins were used for immunization of rabbits.
Fulltext: contact IEB authors
IEB authors: Noemi Čeřovská,
Tomas Moravec,
Helena Plchová
Čeština
English