Research topics
Analysis of TRAIL receptors and Death Receptor 6 (DR6) signaling and elucidation of their roles in proliferation and apoptosis of normal and transformed cells; characterization of involvement of the adaptor protein Daxx in the regulation of cell proliferation and apoptosis in normal and transformed cells.
Current grant support
Ministry of Education, Youth and Sports of the Czech Republic (Center of Molecular and Cellular Immunology, 1M0506)
EC FP6 STREP project (Oncodeath, LSHG-CT-2006-037278)
GA AS CR (Nanomed - KAN200520703, KJB500520801)
GA CR (P301/10/1971)
IGA Ministry of Health (NS10287-3/2009, co-holder)
Most important recent papers
(2005-present; names of the lab members underlined)Drosopoulos KG, Roberts ML, Cermak L, Sasazuki T, Shirasawa S, Andera L, Pintzas A. Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway. J Biol Chem 280(24): 22856-67, 2005. (Pubmed) (DOI)
Tomasetti M, Andera L, Alleva R, Borghi B, Neuzil J, Procopio A. Alpha-tocopheryl succinate induces DR4 and DR5 expression by a p53-dependent route: implication for sensitisation of resistant cancer cells to TRAIL apoptosis. FEBS Lett 580(8): 1925-31, 2006. (Pubmed) (DOI)
Oikonomou E, Kothonidis K, Taoufik E, Probert E, Zografos G, Nasioulas G, Andera L, Pintzas A. Newly established tumourigenic primary human colon cancer cell lines are sensitive to TRAIL-induced apoptosis in vitro and in vivo. Br J Cancer 97(1): 73-84, 2007. (Pubmed) (DOI)
Simova S, Klima M, Cermak L, Sourkova V, Andera L. Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis 13(3): 423-36, 2008. (Pubmed) (DOI)
Doubravska L, Simova S, Cermak L, Valenta T, Korinek V, Andera L. Wnt-expressing rat embryonic fibroblasts suppress Apo2L/TRAIL-induced apoptosis of human leukemia cells. Apoptosis 13(4): 573-87, 2008. (Pubmed) (DOI)
Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermak L, Andera L, Horejsi V, Stockinger H. LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol 45(6): 1703-11, 2008. (Pubmed) (DOI)
Klener P Jr, Leahomschi S, Molinsky J, Simonova T, Necas E, Gasova Z, Cermak J, Dolezalova L, Andera L, Zivny J. TRAIL-induced apoptosis of HL60 leukemia cells: two distinct phenotypes of acquired TRAIL resistance that are accompanied with resistance to TNFalpha but not to idarubicin and cytarabine. Blood Cells Mol Dis 42(1): 77-84, 2009. (Pubmed) (DOI)
Klima M, Zajedova J, Doubravska L, Andera L. Functional analysis of the posttranslational modifications of the death receptor 6. Biochim Biophys Acta 1793(10): 1579-87, 2009. (Pubmed) (DOI)
Oikonomou E, Kosmidou V, Katseli A, Kothonidis K, Mourtzoukou D, Kontogeorgos G, Andera L, Zografos G, Pintzas A. TRAIL receptor upregulation and the implication of KRAS/BRAF mutations in human colon cancer tumors. Int J Cancer 125(9): 2127-35, 2009. (Pubmed) (DOI)
Reviews
Neuzil J, Stantic M, Zobalova R, Chladova J, Wang X, Prochazka L, Dong L, Andera L, Ralph SJ. Tumour-initiating cells vs. cancer 'stem' cells and CD133: what's in the name? Biochem Biophys Res Commun 355(4): 855-9, 2007. (Pubmed) (DOI)
Andera L. Signaling activated by the death receptors of the TNFR family. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 153(3): 173-80, 2009. (Pubmed)
Teaching, lectures, public presentations
MB150P79 - Molecular Mechanisms of Apoptosis - Faculty of Science, Charles University, Prague; presentations for Czech students.