Determination of biological activity and chemical composition of selected tropical and subtropical Ranunculaceae species

Duration: 
01.01.2008 - 31.12.2010
Classification
Grant agency: 
GACR
Grant type: 
Standardní projekt
Registration number: 
GA525/08/1179
Investigators
Internal investigator/coinvestigator: 
Helena Sovová
Internal investigator: 
is coinvestigator only
External investigator/coinvestigator: 
Česká zemědělská univerzita v Praze / Institut tropů a subtropů
Annotation: 

The crude extracts and essential oils, obtained from species of Ranunculaceae family selected according to the phytochemical and ethnobotanical data, will be examined on various biological in vitro assays to evaluate their potential antimicrobial, anti-inflammatory and antioxidative effects in frame of this project. Subsequently, specific active fractions or pure compounds will be isolated and purified using bioassay-guided approach. Traditional analytical (e.g. HPLC, HPLC-MS, GC, GC-MS, NMR) and separation/purification methods (e.g. TLC, CC, MPLC) as well as some modern analytical and separation techniques (e.g. SFE, GCxGC-TOF-MS) will be used to obtain and characterize active fractions or pure compounds.The supposed results of this project could be used by food or pharmaceutical industry for development of new herbal-based nutraceuticals, functional foods, food additives and pharmaceutical or veterinary preparations. GA525/08/1179