RNAi Depletion of Gamma -Tubulin as a Tool for Studying Gamma -Tubulin Role in Organization of Acentrosomal Cells

Doskočilová, Anna; Procházková, Jiřina; Cenklová, Věra; Binarová, Pavla
In Annual Meeting of The American Society for Cell Biology 46. - San Diego: Springer 2006 : 137, 2006

Keywords: microtubules; gamma-tubulin; mitosis
Abstract: We studied the downregulation of Gamma-tubulin in Arabidopsis thaliana by inducible RNAi technique. The RNAi depletion of Gamma-tubulin led to serious distortions of development in A. thaliana seedlings. We observed changes both on the whole plant level and on the microscopical level. The distribution of microtubules was randomized as we expected and the longitudinal growth was impaired. We also observed the alternation of cytokinesis although the cells did not display mitotic changes. This is the field of activity we would like to furhter elucidate
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IEB authors: Pavla Binarová, Věra Cenklová