Hana Šimková, Jan Šafář, Marie Kubaláková, Pavla Suchánková, Jarmila Číhalíková, Heda Robert-Quatre, Perumal Azhaguvel , Yiqun Weng, Junhua H. Peng, Nora L.V. Lapitan, Yaqin Ma, Frank M. You, Ming-Cheng Luo, Jan Bartoš, Jaroslav Doležel
Journal of Biomedicine and Biotechnology
2011:
302543,
2011
Keywords:
Abstract:
Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent
an essential tool for positional cloning. However, wheat BAC libraries comprise more than million clones, which makes their
screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were
constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with
113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb
providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene
DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistance gene Gb3. The small number
of clones combined with high coverage made the screening highly efficient and cost effective.
Fulltext: contact IEB authors
IEB authors: Jan Bartoš,
Jarmila Číhalíková,
Jaroslav Doležel,
Marie Kubaláková,
Heda Robert-Quatre,
Jan Šafář,
Hana Šimková,
Pavla Suchánková
