Illustration of fluorescenct molecules diffusing in and out of the confocal volume
Selected publications dealing with application of FCS in various fields:
Sykora, J., K. Kaiser, I. Gregor, W. Bonigk, G. Schmalzing, and J. Enderlein, Exploring fluorescence antibunching in solution to determine the stoichiometry of molecular complexes. Analytical Chemistry, 2007. 79(11): p. 4040-4049,
Kapusta, P., M. Wahl, A. Benda, M. Hof, and J. Enderlein, Fluorescence lifetime correlation spectroscopy. Journal of Fluorescence, 2007. 17(1): p. 43-48,
Gielen, E., J. Vercammen, J. Sykora, J. Humpolickova, M. vandeVen, A. Benda, N. Hellings, M. Hof, Y. Engelborghs, P. Steels, and M. Ameloot, Diffusion of sphingomyelin and myelin oligodendrocyte glycoprotein in the membrane of OLN-93 oligodendroglial cells studied by fluorescence correlation spectroscopy. Comptes Rendus Biologies, 2005. 328(12): p. 1057-1064,
Benda, A., M. Hof, M. Wahl, M. Patting, R. Erdmann, and P. Kapusta, TCSPC upgrade of a confocal FCS microscope. Review of Scientific Instruments, 2005. 76(3),
Matejicek, P., J. Humpolickova, K. Prochazka, Z. Tuzar, M. Spirkova, M. Hof, and S.E. Webber, Hybrid block copolymer micelles with partly hydrophobically modified polyelectrolyte shells in polar and aqueous media: Experimental study using fluorescence correlation spectroscopy, time-resolved fluorescence, light scattering, and atomic force microscopy. Journal of Physical Chemistry B, 2003. 107(32): p. 8232-8240,
Humpolickova, J., K. Prochazka, M. Hof, Z. Tuzar, and M. Spirkova, Fluorescence correlation spectroscopy using octadecylrhodamine B as a specific micelle-binding fluorescent tag; Light scattering and tapping mode atomic force microscopy studies of amphiphilic water-soluble block copolymer micelles. Langmuir, 2003. 19(10): p. 4111-4119,
Humpolickova, J., K. Prochazka, and M. Hof, Octadecylrhodamine B as a specific micelle-binding fluorescent tag for fluorescence correlation spectroscopy studies of amphiphilic water-soluble block copolymer micelles. Spectroscopic behavior in aqueous media. Collection of Czechoslovak Chemical Communications, 2003. 68(11): p. 2105-2119,
Benes, M., J. Hudecek, P. Anzenbacher, and M. Hof, Coumarin 6, hypericin, resorufins, and flavins: Suitable chromophores for fluorescence correlation spectroscopy of biological molecules. Collection of Czechoslovak Chemical Communications, 2001. 66(6): p. 855-869,
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