Wenzl, P., Suchánková, P., Carling, J., Šimková, H., Huttner, E., Kubaláková, M., Sourdille, P., Paul, E., Feuillet, C., Kilian, A., Doležel, J.
THEORETICAL AND APPLIED GENETICS
121:
465-474,
2010
Keywords:
Abstract:
We describe how the diversity arrays technology
(DArT) can be coupled with chromosome sorting to
increase the density of genetic maps in specific genome
regions. Chromosome 3B and the short arm of chromosome
1B (1BS) of wheat were isolated by flow cytometric
sorting and used to develop chromosome- and chromosome
arm-enriched genotyping arrays containing 2,688 3B
clones and 384 1BS clones. Linkage analysis showed that
553 of the 711 polymorphic 3B-derived markers (78%)
mapped to chromosome 3B, and 59 of the 68 polymorphic
1BS-derived markers (87%) mapped to chromosome 1BS,
confirming the efficiency of the chromosome-sorting
approach. To demonstrate the potential for saturation of
genetic maps, we constructed a consensus map of chromosome
3B using 19 mapping populations, including some
that were genotyped with the 3B-enriched array. The
3B-derived DArT markers doubled the number of genetic
loci covered. The resulting consensus map, probably the
densest genetic map of 3B available to this date, contains
939 markers (779 DArTs and 160 other markers) that
segregate on 304 genetically distinct loci. Importantly, only
2,688 3B-derived clones (probes) had to be screened to
obtain almost twice as many polymorphic 3B markers
(510) as identified by screening approximately 70,000
whole genome-derived clones (269). Since an enriched
DArT array can be developed from less than 5 ng of
chromosomal DNA, a quantity which can be obtained
within 1 h of sorting, this approach can be readily applied
to any crop for which chromosome sorting is available.
Fulltext: contact IEB authors
IEB authors: Jaroslav Doležel,
Marie Kubaláková,
Hana Šimková,
Pavla Suchánková
Čeština
English