Laboratory of Tissue Engineering

Structure of Na⁺/K⁺-ATPase from mouse brain (α2 isoform) in both E1 and E2 conformations.

Structure of the H4-H5 loop of Na⁺/K⁺-ATPase. The N-domain bends toward the P-domain by 64. 8°.

Detailed structure of the phosphorylation site in the E1 and E2 conformations. A hydrogen bond between the O atom of Asp369 and the N atom of Lys690 detected in the E1 conformation disappears in the E2 conformation, accompanied by the appearance of the short π-helix.

Current grant support

GA AS CR, IAA500390702, Scaffolds from liposome nanofibers for tissue engineering, 2007–2011.

Ministry of Education, NPV II 2B06130, Synthesis of new biomaterials and preparation of stem cell derived cells, and their applications in for the treatment of diseases affecting human tissues derived from mesoderm: cartilage, bone, ligament and meniscus, 2006–2011.

EU 7th FP, ID number 214539, Bioscent – Bioactive highly porous and injectable scaffolds controlling stem cells recruitment, proliferation and differenciation and enabling angiogenesis for cardiovascular engineered tissues, 2009–2013.

GA AS CR, Institutional research plan AV0Z50390512, Molecular, cellular and systemic mechanisms of major diseases of the human organism, their diagnosis, therapy and pharmacotherapy, 2005–2011.

GA AS CR, Institutional research plan AV0Z50390703, New biotechnologies, nanomaterials and stem cells for use in regenerative medicine, 2007–2013.

Patent

Patent No. 300142 – The way of preparation of fibrous biodegradable tectorial materials as drug carriers with controlled delivery. Industrial Property Office (Czech Patent and Trademark Office), 25. 2. 2009, Filing Date: 22. 3. 2006.

Selected recent publications

1. Filová E, Rampichová M, Handl M, Lytvynets A, Halouzka R, Usvald D, Hlučilová J, Procházka R, Dezortová M, Rolencová E, Košťáková E, Trč T, Šťastný E, Koláčná L, Hájek M, Motlík J, Amler E. (2007) Composite hyaluronate-type I collagen-fibrin scaffold in the therapy of osteochondral defects in miniature pigs. Physiol Res 56 Suppl. 1., S5–S16. PDF file (12.6 MB)

2. Koláčná L, Bakešová J, Varga F, Košťáková E, Plánka L, Nečas A, Lukáš D, Amler E, Pelouch V. (2007) Biochemical and biophysical aspects of collagen nanostructure in the extracellular matrix. Physiol Res 56 Suppl. 1., S51–S60. PDF file (617 kB)

3. Varga F, Držík M, Handl M, Chlpík J, Kos P, Filová E, Rampichová M, Nečas A, Trč T, Amler E. (2007) Biomechanical characterization of cartilages by a novel approach of blunt impact testing. Physiol Res 56 Supl. 1., S61–S68. PDF file (772 kB)

4. Míčková A, Tománková K, Kolářová H, Bajgar R, Kolář P, Šunka P, Plencner M, Jakubová R, Beneš J, Koláčná L, Plánka L, Nečas A, Amler E. (2008). Ultrasonic shock-wave as a control mechanism for liposome drug delivery system for possible use in scaffold implanted to animals with iatrogenic articular cartilage defects. Acta Vet Brno 77: 285–289. PDF file (1.1 MB)

5. Tejral G, Koláčná L, Schoner W, Amler E. (2008). The π-helix formation between Asp369 and Thr375 as a key factor in E1-E2 conformational change of Na⁺/K⁺-ATPase. Physiol Res in press PDF file (492 kB)

6. Filová E, Jelínek F, Handl M, Lytvynets A, Rampichová M, Varga F, Činátl J, Soukup T, Trč T, Amler E. (2008) Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee. J Biomed Mater Res B Appl Biomater 87B(2): 415–424. PDF file (480 kB)

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