Home › Research › Laboratories › Molecular Cytogenetics and Cytometry

Laboratory of Molecular Cytogenetics and Cytometry

Contacts
Group head: 
Jaroslav Doležel
Centre: 
Olomouc – Sokolovská
Address: 
Sokolovská 6, 772 00 Olomouc
Email: 
dolezelatueb [dot] cas [dot] cz
Phone: 
+420 585 205 852
Fax: 
+420 585 205 853
www: 
Laboratory of Molecular Cytogenetics and Cytometry

Research Topics

The Laboratory of Molecular Cytogenetics and Cytometry has been established to study the structure and function of nuclear genome in higher plants. The main focus is on economically important crops like cereals, legumes, bananas and plantains.

Methodological approaches involve classical and molecular cytogenetics, flow cytometry and sorting, recombinant DNA techniques and construction of chromosome and chromosome-arm specific BAC libraries. Because of the complexity of research projects, the Laboratory collaborates with many research groups throughout the world. The laboratory staff is also involved in teaching and technology transfer, including consultancy services in the above mentioned areas.

Flow cytogenetics

Flow cytogenetics involve chromosome analysis and sorting using flow cytometry applications of sorted chromosomes include physical mapping using PCR, targeted isolation of molecular markers, isolation of low-copy (genic) sequences from specific genome regions, high-throughput physical mapping of DNA sequences by hybridization to DNA arrays, as well as HAPPY and optical mapping.

Chromosome-based BAC libraries recources

The most attractive use of sorted chromosomes is the production of chromosome- and chromosome arm-specific DNA libraries. Such libraries provide a permanent and easily accessible molecular resource specific for a particular part of the genome, is expected to have a major impact on wheat genomics.

Flow cytometry

Flow cytometry is powerful technique with many application. We used it for genome size estimation, ploidy measurement and flow karyotype analysis of higher plants.

Banana studies

We interested in molecular evolution and organization of Musa genome and serve as the Musa Genome Resources Centre (MGRC). The primary aim of the Centre is to support research activities of the Musa Genomics Consortium by distributing Musa genome resources to Consortium Members and by developing new resources.

Study on Festuca-Lolium complex

We are using molecular cytogenetic methods (FISH and GISH) to study genomic constitution of Festuca × Lolium hybrids, chromosome pairing during meiosis, and to map BAC clones on chromosomes of Festuca pratensis. In collaboration with other labs, we also developed set of introgression lines of all F. pratensis chromosomes in tetraploid L. multiflorum and DArT chip for five species within Festuca-Lolium complex.

Workers

Workers: 

scientist

postgraduate student

graduated technical assistant

head of the laboratory
senior scientist

technician

postdoc

Publications

Publications: 
Number of publications: 132

2008