Research topics
Immunoregulation, transplantation immunity, stem cells, regulatory T cells, cytokines and other immunoregulatory molecules, gene expression.
Current grant support
Grant Agency of the Czech Republic Nos. 306/08/0640, 310/08/H077, P304/11/0653 and P301/11/1568.
Grant Agency of the Ministry of Education No. 1M0506
Grant Agency of the Academy of Science project No. KAN200520804
Most important recent papers
(2006-present; names of the lab members underlined)Holan V, Pindjakova J, Krulova M, Neuwirth A, Fric J, Zajicova A. Production of nitric oxide during graft rejection is regulated by the Th1/Th2 balance, the arginase activity, and L-arginine metabolism. Transplantation 81(12): 1708-15, 2006. (Pubmed) (DOI)
Kubera M, Roman A, Basta-Kaim A, Budziszewska B, Zajicova A, Holan V, Rogoz Z, Skuza G, Leskiewicz M, Regulska M, Jagla G, Nowak W, Lason W. Effect of acute and repeated treatment with mirtazapine on the immunity of noradrenaline transporter knockout C57BL/6J mice. Pharmacol Biochem Behav 85(4): 813-9, 2006. (Pubmed) (DOI)
Tavandzi U, Prochazka R, Usvald D, Hlucilova J, Vitaskova M, Motlik J, Vitova A, Filipec M, Forrester JV, Holan V. A new model of corneal transplantation in the miniature pig: efficacy of immunosuppressive treatment. Transplantation 83(10): 1401-3, 2007. (Pubmed) (DOI)
Pialek J, Vyskocilova M, Bimova B, Havelkova D, Pialkova J, Dufkova P, Bencova V, Dureje L, Albrecht T, Hauffe HC, Macholan M, Munclinger P, Storchova R, Zajicova A, Holan V, Gregorova S, Forejt J. Development of unique house mouse resources suitable for evolutionary studies of speciation. J Hered 99(1): 34-44, 2008. (Pubmed) (DOI)
Fric J, Marek M, Hruskova V, Holan V, Forstova J. Cellular and humoral immune responses to chimeric EGFP-pseudocapsids derived from the mouse polyomavirus after their intranasal administration. Vaccine 26(26): 3242-51, 2008. (Pubmed) (DOI)
Krulova M, Pokorna K, Lencova A, Fric J, Zajicova A, Filipec M, Forrester JV, Holan V. A rapid separation of two distinct populations of mouse corneal epithelial cells with limbal stem cell characteristics by centrifugation on percoll gradient. Invest Ophthalmol Vis Sci 49(9): 3903-8, 2008. (Pubmed) (DOI)
Prochazkova J, Fric J, Pokorna K, Neuwirth A, Krulova M, Zajicova A, Holan V. Distinct regulatory roles of transforming growth factor-beta and interleukin-4 in the development and maintenance of natural and induced CD4+ CD25+ Foxp3+ regulatory T cells. Immunology 128(1 Suppl): e670-8, 2009. (Pubmed) (DOI)
Lipoldova M, Havelkova H, Badalova J, Vojtiskova J, Quan L, Krulova M, Sohrabi Y, Stassen AP, Demant P. Loci controlling lymphocyte production of interferon c after alloantigen stimulation in vitro and their co-localization with genes controlling lymphocyte infiltration of tumors and tumor susceptibility. Cancer Immunol Immunother 59(2): 203-13, 2010. (Pubmed) (DOI)
Furugaki K, Pokorna K, Le Pogam C, Aoki M, Reboul M, Bajzik V, Krief P, Janin A, Noguera ME, West R, Charron D, Chomienne C, Pla M, Moins-Teisserenc H, Padua RA. DNA vaccination with all-trans retinoic acid treatment induces long-term survival and elicits specific immune responses requiring CD4+ and CD8+ T-cell activation in an acute promyelocytic leukemia mouse model. Blood 115(3): 653-6, 2010. (Pubmed) (DOI)
Holan V, Pokorna K, Prochazkova J, Krulova M, Zajicova A. Immunoregulatory properties of mouse limbal stem cells. J Immunol 184(4): 2124-9, 2010. (Pubmed) (DOI)
Zajicova A, Pokorna K, Lencova A, Krulova M, Svobodova E, Kubinova S, Sykova E, Pradny M, Michalek J, Svobodova J, Munzarova M, Holan V. Treatment of ocular surface injuries by limbal and mesenchymal stem cells growing on nanofiber scaffolds. Cell Transplant 19(10): 1281-90, 2010. (Pubmed) (DOI)
Kankova S, Holan V, Zajicova A, Kodym P, Flegr J. Modulation of immunity in mice with latent toxoplasmosis--the experimental support for the immunosuppression hypothesis of Toxoplasma-induced changes in reproduction of mice and humans. Parasitol Res 107(6): 1421-7, 2010. (Pubmed) (DOI)
Lencova A, Pokorna K, Zajicova A, Krulova M, Filipec M, Holan V. Graft survival and cytokine production profile after limbal transplantation in the experimental mouse model. Transpl Immunol 24(3): 189-94, 2011. (Pubmed) (DOI)
Svobodova E, Krulova M, Zajicova A, Pokorna K, Prochazkova J, Trosan P, Holan V. The Role of Mouse Mesenchymal Stem Cells in Differentiation of Naive T-Cells into Anti-Inflammatory Regulatory T-Cell or Proinflammatory Helper T-Cell 17 Population. Stem Cells Dev. 2011 Jul 26. [Epub ahead of print] (Pubmed) (DOI)
Holan V, Chudickova M, Trosan P, Svobodova E, Krulova M, Kubinova S, Sykova E, Sirc J, Michalek J, Juklickova M, Munzarova M, Zajicova A. Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression. J Control Release. 2011 Jul 23. [Epub ahead of print] (Pubmed) (DOI)
Reviews
Holan V. Corneal stromal cells selectively inhibit the production of certain anti-inflammatory cytokines. Expert Rev. Clin. Immunol. 2: 101-8, 2006.
Teaching, lectures, public presentations
MB150P13 - Regulatory Mechanisms of Immunity - Faculty of Science, Charles University, Prague; semestral course in Czech.