The aim of the project is to apply supercritical fluid extraction, an advanced "green" technology, to isolate biologically active substances that are simultaneously efficient against insects and nontoxic for vertebrates from selected plants. The projectincludes plant screening, research on the methods enhancing production of biologically active substances in plants, extensive biological tests of extract effects on insects, and optimisation of extraction conditions in a wide range with the aim to concentrate the biologically active substances in the extracts. The results of the project will be applied to develop safe, highly efficient, and stable natural crop protectants and/or insecticides enabling planting crops for high-quality health foods. As medicinal and spice plants are among the examined botanical materials, the results of the project may be utilised also in the production of pharmaceuticals and nutraceuticals. 2B06049